权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

An advanced integrative mass spectrometer - the essential tool for in-depth analysis of diverse biological research.

先进的综合质谱仪 - 深入分析各种生物研究的必备工具。

基本信息

批准号：
BB/T017686/1
负责人：
Sally Shirran
金额：
$ 88.15万
依托单位：
University of St Andrews
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2020
资助国家：
英国
起止时间：
2020 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FT017686%2F1
关键词：
advanced integrative mass spectrometer essential

项目摘要

Mass spectrometry is an analysis technique that measures the molecular weight of a molecule, and gives information about its elemental composition. This technique became applicable to biomolecules by the introduction of soft ionisation techniques. Here the intact molecule can be measured and then subsequently fragmented into its component parts. The molecular weight of those parts can be determined, which gives further information about the molecular structure, and the data collectively allows the full identification of the molecule to help answer important biological questions. The development and application of mass spectrometry in the biological field has expanded rapidly over the last few decades and mass spectrometry is now established as a cornerstone of biological analysis. The growth in applications has stemmed from ever increasing gains in resolution and accuracy. A step-change in resolution and accuracy was enabled by the development of the orbitrap mass analyser, which has become an essential instrument in the modern bioanalytical environment. The Biomedical Sciences Research Complex is a multidisciplinary centre that carries out internationally recognised science at the interface of biology, chemistry, physics and medicine. The mass spectrometry core facility underpins much of this research. However, the current instrumentation is limited and unfortunately some experiments cannot be conducted due to these limitations. This hinders the research and leaves some scientific questions unanswered. We are therefore applying for a Fusion Lumos Orbitrap mass spectrometer with Vanquish UHPLC.This high end, versatile mass spectrometer will transform a wide range of BBSRC-funded projects in St Andrews and across the UK. The special configuration of the instrument with 3 mass analysers and multiple ways of fragmenting the molecules, alongside the exceptional resolution, will revolutionalise our ability to analyse biomolecules and have a massive impact on the research that we can support, far beyond the boundaries of what we can currently offer with our instrumentation. Researchers from universities and research institutions from across the UK access the facility, and the facility additionally collaborates with industry supporting commercial research, so the benefits will be far reaching, ultimately strengthening our biotechnology and biological research.The research in this proposal is diverse and encompasses all areas of the BBSRC remit. The applications of the mass spectrometer, and the numerous ways it can benefit the various research projects are equally diverse and impacts all areas of high-end life science research. The versatility of the technique in analysing, identifying and even quantifying a diverse range of molecules from large biomolecules such as proteins and DNA, to small peptides and metabolites, has seen the demand for mass spectrometry analysis expand rapidly. The information derived from a mass spectrometric analysis can be used to define differences in composition and structure of two states of biomolecules and can lead to novel insights into the molecules' role and function. Understanding these aspects of biomolecules is fundamental to research into disease, the ageing process, food security, nutrition, cancer, pathogens, anti-microbial resistance, fundamental biological processes and synthetic biology; several of which are key strategic areas priorities for the BBSRC.

质谱仪是一种分析技术，它测量分子的相对分子质量，并提供有关其元素组成的信息。通过引入软电离技术，这项技术变得适用于生物分子。在这里，可以测量完整的分子，然后将其裂解成其组成部分。可以确定这些部分的分子量，这提供了关于分子结构的进一步信息，这些数据共同允许全面识别分子，以帮助回答重要的生物学问题。在过去的几十年里，质谱学在生物领域的发展和应用得到了迅速的发展，现在质谱学已经成为生物分析的基石。应用程序的增长源于分辨率和准确性的不断提高。Orbitrap质量分析仪的开发使分辨率和准确度发生了阶段性的变化，该仪器已成为现代生物分析环境中的重要仪器。生物医学科学研究中心是一个多学科中心，在生物、化学、物理和医学领域开展国际公认的科学研究。质谱学核心设备支撑了这项研究的大部分内容。然而，目前的仪器是有限的，不幸的是，由于这些限制，一些实验无法进行。这阻碍了研究，并留下了一些科学问题没有得到回答。因此，我们正在申请一台带有Vanquish UHPLC的Fusion Lumos Orbitrap质谱仪。这款高端、多功能的质谱仪将改变圣安德鲁斯和整个英国由BBSRC资助的一系列项目。该仪器的特殊配置具有3个质量分析器和多种分子裂解方法，以及卓越的分辨率，将彻底改变我们分析生物分子的能力，并对我们可以支持的研究产生巨大影响，远远超出我们目前仪器所能提供的范围。来自英国各地大学和研究机构的研究人员可以使用该设施，该设施还与支持商业研究的行业合作，因此好处将是深远的，最终将加强我们的生物技术和生物研究。这项提议中的研究是多样化的，涵盖了BBSRC职权范围的所有领域。质谱仪的应用以及它可以使各种研究项目受益的许多方式都是同样多样化的，并影响着高端生命科学研究的所有领域。这项技术在分析、鉴定甚至量化各种分子方面的多功能性，从蛋白质和DNA等大的生物分子，到小肽和代谢物，都见证了对质谱分析的需求迅速扩大。来自质谱分析的信息可以用来定义两种状态的生物分子在组成和结构上的差异，并可能导致对分子的作用和功能的新见解。了解生物分子的这些方面对于研究疾病、衰老过程、食品安全、营养、癌症、病原体、抗微生物耐药性、基本生物学过程和合成生物学至关重要；其中几个领域是BBSRC的关键战略优先领域。