Integrative Omics Facility Core

综合组学设施核心

• 批准号: 10610208
• 负责人: George S Watts
• 金额: $ 15.83万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10610208
• 关键词: Address; Air; Applications Grants; Arsenic; Awareness; Biological; Biological Markers; Budgets; Chemicals; Computers and Advanced Instrumentation; Consult; Consultations; Core Facility; DNA Methylation; DNA sequencing; Data; Data Analyses; Dideoxy Chain Termination DNA Sequencing; Disease; Disulfide Linkage; Dust; Ensure; Environment; Environmental Health; Equipment; Experimental Designs; Genome; Genomics; Genotype; Goals; Health Sciences; Histones; Individual; Industrialization; Information Dissemination; Institution; Investments; Laboratories; Lead; Logistics; Manuscripts; Maps; Mass Spectrum Analysis; Measurement; Measures; Mercury; Metals; Methods; Mission; Modeling; Modernization; Peptides; Persons; Plants; Population; Post-Translational Protein Processing; Preparation; Process; Proteins; Proteome; Proteomics; Quality Control; Research; Research Design; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Risk; Sampling; Scientist; Secure; Services; Site; Students; Teacher Professional Development; Techniques; Technology; Time; Tissues; Toxic Environmental Substances; Training and Education; Trichloroethylene; Universities; Vision; Water; cost effective; cost efficient; epigenome; epigenomics; equipment acquisition; exome; experience; experimental study; health science research; innovation; insight; instrumentation; member; metabolome; metabolomics; monomethylarsonic acid; pilot test; technique development; technology/technique; toxicant; transcriptome; transcriptome sequencing; whole genome

The Omics Facility Core (OFC) assists members in fulfilling the mission of SWEHSC to understand the mechanisms underlying environmental health science (EHS) risks and disease among people living in arid environments by providing high-quality, cost-efficient access to knowledgeable scientists and a full spectrum of modern technologies and techniques. The technologies and techniques within the OFC are used to measure environmental toxicants (exposomics) and their effects on the proteome, metabolome, epigenome, and genome of exposed populations and laboratory models. The OFC is composed of three labs: 1) an exposomic lab providing services to quantitate organic and inorganic toxicants of relevance from diverse matrices such as water, air, dust, bodily tissues, and plants; 2) the proteomics lab providing protein identification, mapping post- translational modifications, disulfide linkage characterization, peptide sequencing, and biomarker quantitation; and 3) a genomics lab providing next-gen DNA sequencing of whole genomes, exomes, and transcriptomes (RNA-seq), real time RT-PCR, genotyping, fragment analysis, and Sanger sequencing. Finally, epigenomic and metabolomic services including analysis of histone protein modifications, DNA methylation, and quantitation of toxicant metabolites are distributed across the three Omics laboratories based on the analyte and expertise required. The OFC provides Center investigators with prioritized access to state-of-the-art equipment and approaches to achieve the environmental health science (EHS) goals of the SWEHSC. The three Co-Directors of the OFC component labs guide the choice of appropriate analysis, consult on experimental design, manage the analyses and related budget, provide logistical support, provide data interpretation and insight into results, and assist with dissemination of results. Further, the OFC provides training and education to Center investigators' staff and students to 1) provide access to modern analytic techniques, 2) consult in experimental design and efficient use of resources, 3) ensure services are current through technique development and new equipment acquisition, 4) provide data analysis and interpretation, and 5) assist in the preparation of manuscripts and grant applications. Integration with other SWEHSC resources facilitates the acquisition of omics-level data and information that are necessary to fully determine the sites and modes of toxicant action.

组学设施核心（OFC）协助成员履行SWEHSC的使命， 干旱和半干旱地区人民的环境健康科学风险和疾病的潜在机制 通过提供高质量，具有成本效益的知识渊博的科学家和全方位的 现代技术和工艺。OFC中的技术和方法用于测量 环境毒物（暴露组学）及其对蛋白质组、代谢组、表观基因组和基因组的影响 暴露人群和实验室模型。OFC由三个实验室组成：1）生物学实验室 提供服务，从各种基质中定量相关的有机和无机毒物， 水，空气，灰尘，身体组织和植物; 2）蛋白质组学实验室提供蛋白质鉴定， 翻译修饰、二硫键表征、肽测序和生物标志物定量; 以及3）基因组学实验室，提供全基因组、外显子组和转录组的下一代DNA测序 （RNA-seq）、真实的时间RT-PCR、基因分型、片段分析和桑格测序。最后，表观基因组和 代谢组学服务，包括分析组蛋白蛋白修饰，DNA甲基化和定量 根据分析物和专业知识，有毒代谢物分布在三个组学实验室中 必需的. OFC为中心调查人员提供优先使用最先进设备的机会， 实现SWEHSC环境健康科学（EHS）目标的方法。三位联合董事 OFC组件实验室的工作人员指导选择合适的分析方法，为实验设计提供咨询， 分析和相关预算，提供后勤支持，提供数据解释和对结果的深入了解， 并协助传播成果。此外，OFC还为中心的研究人员提供培训和教育， 工作人员和学生1）提供现代分析技术，2）在实验设计咨询， 有效利用资源，3）通过技术开发和新设备确保服务是最新的 采购，4）提供数据分析和解释，以及5）协助准备手稿和赠款 应用.与其他SWEHSC资源的集成促进了omics级数据的获取， 充分确定毒物作用的地点和方式所必需的信息。