Integrative Omics Facility Core

综合组学设施核心

• 批准号: 10610208
• 负责人: George S Watts
• 金额: $ 15.83万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10610208
• 关键词: Address; Air; Applications Grants; Arsenic; Awareness; Biological; Biological Markers; Budgets; Chemicals; Computers and Advanced Instrumentation; Consult; Consultations; Core Facility; DNA Methylation; DNA sequencing; Data; Data Analyses; Dideoxy Chain Termination DNA Sequencing; Disease; Disulfide Linkage; Dust; Ensure; Environment; Environmental Health; Equipment; Experimental Designs; Genome; Genomics; Genotype; Goals; Health Sciences; Histones; Individual; Industrialization; Information Dissemination; Institution; Investments; Laboratories; Lead; Logistics; Manuscripts; Maps; Mass Spectrum Analysis; Measurement; Measures; Mercury; Metals; Methods; Mission; Modeling; Modernization; Peptides; Persons; Plants; Population; Post-Translational Protein Processing; Preparation; Process; Proteins; Proteome; Proteomics; Quality Control; Research; Research Design; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Risk; Sampling; Scientist; Secure; Services; Site; Students; Teacher Professional Development; Techniques; Technology; Time; Tissues; Toxic Environmental Substances; Training and Education; Trichloroethylene; Universities; Vision; Water; cost effective; cost efficient; epigenome; epigenomics; equipment acquisition; exome; experience; experimental study; health science research; innovation; insight; instrumentation; member; metabolome; metabolomics; monomethylarsonic acid; pilot test; technique development; technology/technique; toxicant; transcriptome; transcriptome sequencing; whole genome

The Omics Facility Core (OFC) assists members in fulfilling the mission of SWEHSC to understand the mechanisms underlying environmental health science (EHS) risks and disease among people living in arid environments by providing high-quality, cost-efficient access to knowledgeable scientists and a full spectrum of modern technologies and techniques. The technologies and techniques within the OFC are used to measure environmental toxicants (exposomics) and their effects on the proteome, metabolome, epigenome, and genome of exposed populations and laboratory models. The OFC is composed of three labs: 1) an exposomic lab providing services to quantitate organic and inorganic toxicants of relevance from diverse matrices such as water, air, dust, bodily tissues, and plants; 2) the proteomics lab providing protein identification, mapping post- translational modifications, disulfide linkage characterization, peptide sequencing, and biomarker quantitation; and 3) a genomics lab providing next-gen DNA sequencing of whole genomes, exomes, and transcriptomes (RNA-seq), real time RT-PCR, genotyping, fragment analysis, and Sanger sequencing. Finally, epigenomic and metabolomic services including analysis of histone protein modifications, DNA methylation, and quantitation of toxicant metabolites are distributed across the three Omics laboratories based on the analyte and expertise required. The OFC provides Center investigators with prioritized access to state-of-the-art equipment and approaches to achieve the environmental health science (EHS) goals of the SWEHSC. The three Co-Directors of the OFC component labs guide the choice of appropriate analysis, consult on experimental design, manage the analyses and related budget, provide logistical support, provide data interpretation and insight into results, and assist with dissemination of results. Further, the OFC provides training and education to Center investigators' staff and students to 1) provide access to modern analytic techniques, 2) consult in experimental design and efficient use of resources, 3) ensure services are current through technique development and new equipment acquisition, 4) provide data analysis and interpretation, and 5) assist in the preparation of manuscripts and grant applications. Integration with other SWEHSC resources facilitates the acquisition of omics-level data and information that are necessary to fully determine the sites and modes of toxicant action.

OMICS设施核心（OFC）可帮助成员履行SWEHSC的使命了解 居住在干旱的人中的环境健康科学（EHS）风险和疾病的机制 通过为知识渊博的科学家提供高质量，成本效益的访问和全面的环境 现代技术和技术。 OFC内的技术和技术用于测量 环境有毒物质（外汇学）及其对蛋白质组，代谢组，表观基因组和基因组的影响 裸露的人群和实验室模型。 OFC由三个实验室组成：1）爆炸性实验室 提供服务来量化各种矩阵的相关性有机和无机有毒物质 水，空气，灰尘，身体组织和植物； 2）提供蛋白质鉴定的蛋白质组学实验室，在 - 翻译修饰，二硫键特征，肽测序和生物标志物定量； 3）提供整个基因组，异构体和转录组的下一代DNA测序的基因组学实验室 （RNA-SEQ），实时RT-PCR，基因分型，片段分析和Sanger测序。最后，表观基因组和 代谢组学服务包括组蛋白蛋白质修饰，DNA甲基化和定量的分析 有毒代谢物分布在三个基于分析的OMIC实验室中 必需的。 OFC为中心调查人员提供了优先使用最先进设备的访问权限和 实现SWEHSC环境健康科学（EHS）目标的方法。三个联合导演 OFC组件实验室的指导选择适当的分析，请咨询实验设计，管理 分析和相关预算，提供后勤支持，提供数据解释并了解结果， 并协助传播结果。此外，OFC提供了培训和教育，以中心调查员的中心 员工和学生至1）提供现代分析技术的访问，2）咨询实验设计和 有效利用资源，3）确保通过技术开发和新设备确保服务是当前的 收购，4）提供数据分析和解释，5）协助准备手稿和赠款 申请。与其他SWEHSC资源集成有助于获取OMICS级数据和 充分确定有毒作用的地点和模式所需的信息。