A Proteomics Research Resource for Integrative Biology

综合生物学蛋白质组学研究资源

• 批准号: 8301803
• 负责人: RICHARD D SMITH
• 金额: $ 292万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8301803
• 关键词: Advanced Development; Area; Automation; Beds; Bioinformatics; Biological; Biological Markers; Biology; Biomedical Research; Blood capillaries; Cell Cycle; Cells; Chronic; Communities; Computer software; Computers and Advanced Instrumentation; Country; Coupled; Data; Data Analyses; Data Quality; Department of Energy; Development; Disease; Effectiveness; Environment; Evaluation; Health; Human; Infection; Informatics; Institution; Investigation; Investments; Kidney Transplantation; Laboratories; Mammary gland; Measurement; Measures; Membrane; Methods; Modification; Molecular; Nature; Pacific Northwest; Pathogenicity; Preparation; Process; Productivity; Protein Dynamics; Proteins; Proteome; Proteomics; Publications; Radiation; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Science; Scientist; Signal Pathway; Signal Transduction; Software Tools; Subcellular Fractions; Technical Expertise; Techniques; Technology; Testing; Time; Tissues; Training; Trauma Research; United States National Institutes of Health; Virulent; base; capillary; cell motility; collaboratory; computerized data processing; falls; improved; insight; instrumentation; intercellular communication; mass spectrometer; microbial; nanoscale; new technology; novel strategies; oncology; operation; response; sound; success; technology development; tool; ultra high pressure

The ability to precisely and accurately measure levels of nearly all expressed proteins and their modified forms can provide new insights into the molecular nature and operation of cells, cell signaling pathways and networks, the cell cycle, cellular differentiation, and other processes relevant to human health and to the progression of various disease states. This ?4i Resource Center is focused on serving the NIH-supported biomedical research community by developing and integrating new proteomic technologies for biological applications, disseminating the new technologies, and training scientists in their use. In its first 4 years the Resource Center has cultivated a strong team of researchers, who have achieved a strong publication record, a productive set of collaborative projects, and provided effective dissemination of an array of technology and informatics developments. As a result the Center is now poised for even greater successes. The primary technology objectives of the Resource Center Cores are to develop and apply an integrated set of biological methods, new analytical technologies, and associated computational and informatics tools, for much more rapid, quantitative, sensitive, and comprehensive proteomics measurement applications than presently possible. The three technological Cores of the Resource Center aim at development of: i) improved technologies for spatial and temporal proteomics, which includes approaches to better define the compartment localization of proteins, primarily with respect to membrane compartments and to quantify the dynamics of proteins between compartments; 2) higher throughput, more sensitive, and much more robust quantitative measurements of proteomes that provide broader dynamic range and greater coverage of proteins and their modifications; and 3) computational and bioinformatics tools needed to provide information based on data with statistically sound measures of quality, so as to facilitate new biological understandings. The Center's technology developments will be evaluated in the context of a range of challenging collaborative applications selected on the basis of their scientific and biomedical relevance, as well as their capacity to benefit from the Resource Center's capabilities. The scope of applications include areas of microbial pathogenicity, trauma research, cellular migration, kidney transplantation, signal transduction, neuroproteomics, radiation response, chronic virulent infections, biomarker discovery, and mammary oncology.

精确和准确地测量几乎所有表达的蛋白质及其表达水平的能力， 修饰的形式可以为细胞的分子性质和运作，细胞信号传导， 途径和网络，细胞周期，细胞分化和其他与人类相关的过程 健康和各种疾病状态的进展。这个吗4 i资源中心专注于为 NIH支持的生物医学研究社区通过开发和整合新的蛋白质组 生物应用技术，传播新技术，培训科学家， 他们的使用。在最初的4年里，资源中心培养了一支强大的研究团队，他们 取得了良好的出版记录，一系列富有成效的合作项目，并提供了有效的 传播一系列技术和信息学发展成果。因此，该中心目前 准备取得更大的成功。 资源中心核心的主要技术目标是开发和应用集成的 一套生物学方法，新的分析技术，以及相关的计算和信息学 工具，用于更快速，定量，灵敏和全面的蛋白质组学测量 比目前可能的应用。资源中心的三大技术核心旨在 发展：㈠改进空间和时间蛋白质组学技术，包括各种方法 为了更好地定义蛋白质的区室定位，主要是相对于膜 隔室并量化隔室之间蛋白质的动力学; 2）更高的通量， 更灵敏、更可靠的蛋白质组定量测量， 蛋白质及其修饰的动态范围和更大的覆盖范围;以及3）计算和生物信息学 提供基于数据的信息所需的工具， 以促进新的生物学理解。 该中心的技术发展将在一系列具有挑战性的背景下进行评估， 根据其科学和生物医学相关性以及其 从资源中心的能力中受益的能力。应用范围包括以下领域 微生物致病性，创伤研究，细胞迁移，肾移植，信号转导， 神经蛋白质组学，辐射反应，慢性毒性感染，生物标志物发现，和乳腺癌 肿瘤学

项目成果

Characterization of intact N- and O-linked glycopeptides using higher energy collisional dissociation.

• DOI: 10.1016/j.ab.2014.01.003
• 发表时间: 2014-05-01
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: Cao L;Tolić N;Qu Y;Meng D;Zhao R;Zhang Q;Moore RJ;Zink EM;Lipton MS;Paša-Tolić L;Wu S
• 通讯作者: Wu S

Highly stable trypsin-aggregate coatings on polymer nanofibers for repeated protein digestion.

• DOI: 10.1002/pmic.200800591
• 发表时间: 2009-04
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Kim, Byoung Chan;Lopez-Ferrer, Daniel;Lee, Sang-Mok;Ahn, Hye-Kyung;Nair, Sujith;Kim, Seong H.;Kim, Beom Soo;Petritis, Konstantinos;Camp, David G.;Grate, Jay W.;Smith, Richard D.;Koo, Yoon-Mo;Gu, Man Bock;Kim, Jungbae
• 通讯作者: Kim, Jungbae

Two-dimensional ion mobility analyses of proteins and peptides.

蛋白质和肽的二维离子淌度分析。

• DOI: 10.1007/978-1-59745-493-3_26
• 发表时间: 2009
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Shvartsburg,AlexandreA;Tang,Keqi;Smith,RichardD
• 通讯作者: Smith,RichardD

Monolithic integration of two-dimensional liquid chromatography-capillary electrophoresis and electrospray ionization on a microfluidic device.

• DOI: 10.1021/ac102437z
• 发表时间: 2011-02-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Chambers, Andrew G.;Mellors, J. Scott;Henley, W. Hampton;Ramsey, J. Michael
• 通讯作者: Ramsey, J. Michael

Evaluation of a high-intensity focused ultrasound-immobilized trypsin digestion and 18O-labeling method for quantitative proteomics.

• DOI: 10.1021/ac802540s
• 发表时间: 2009-08-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Lopez-Ferrer, Daniel;Hixson, Kim K.;Smallwood, Heather;Squier, Thomas C.;Petritis, Konstantinos;Smith, Richard D.
• 通讯作者: Smith, Richard D.