Integrative Molecular Analysis Core

集成分子分析核心-项目总结 在快速变化的生物学和医学金属领域，研究需求是多样化和复杂的。 目前，无机生物化学的进展-及其在生物医学现象中的应用-需要 分子分析的复杂集成，包括识别和定量痕量金属的能力 物种形成以及金属与生物分子靶点的相互作用。这些分析需要训练有素的人员 拥有专业知识和先进的高端设备，超越了通常在 大多数独立的研究实验室。整合分子分析核心（IMAC）提供UNM 中心金属在生物学和医学研究人员与必要的专业知识和工具，为各种 现代分子分析，包括ICP-MS，EPR自旋捕获和定量质谱。 此外，核心提供内部分析，支持创新的协议开发，并提供 培训学生，研究员和教师在与先进的分析相关的综合应用程序 IMAC设施中存在的仪器。核心还利用了对其他几个现有的 校园内的核心设施，使创新的生物分子方法，并提供这些服务， 补贴价格。比获得技术更重要的是，IMAC提供量身定制的专业知识， 研究人员协助设计分子分析和数据解释，以优化使用有价值的 生物样品和资源。该设施将由一批专家管理， 分子分析、研究设施管理、培训和指导方面的补充专业知识， 初级教员。核心设备主要安置在靠近大多数用户的专用空间内。 这种资源和知识的集中化提供了整合的、具有成本效益的分析服务 向中心成员和用户提供专业知识和技术。核心由三个单元组成：金属 分析化学、氧化应激和生物质谱。该核心的具体目标是： 1)创建一个集中的、最先进的资源，为以下方面提供专业知识、培训和工具： 综合分子分析，侧重于金属与生物系统的相互作用; 2）支持 指导PI的研究目标，并在中心内培养一批新的COBRE研究人员， 生物学和医学中的金属;以及3）建立一个用户基础， 核心设施的增长和可持续性。预期成果包括加强多学科 支持指导PI的金属项目走向R 01级资金的研究能力， 并增加了UNM内部、新墨西哥州和其他IDEA州的合作。这些成果将 积极影响UNM的卓越研究水平，并加强对新疗法的研究 金属介导的疾病折磨着新墨西哥州和全世界数百万人。

INTEGRATIVE MOLECULAR ANALYSIS CORE – PROJECT SUMMARY In the rapidly changing field of Metals in Biology and Medicine, research needs are diverse and complex. Currently, advances in inorganic biochemistry – and its application to biomedical phenomena – require sophisticated integration of molecular analyses, including the ability to identify and quantitate trace metal speciation and metal interactions with biomolecular targets. These analyses require highly trained personnel with specialized expertise and access to advanced high-end equipment, beyond what is typically available in most individual research laboratories. The Integrative Molecular Analysis Core (IMAC) provides the UNM Center for Metals in Biology and Medicine investigators with the necessary expertise and tools for a variety of modern molecular analyses, including ICP-MS, EPR spin-trapping, and quantitative mass spectrometry. Moreover, the Core provides in-house analyses, supports innovative protocol development, and provides training to students, fellows, and faculty in the integrated applications associated with the advanced analytical instrumentation present in the IMAC facility. The Core additionally leverages access to several other existing on-campus core facilities to enable innovative biomolecular methods, and provides these services at subsidized pricing. Even more important than access to the technology, the IMAC provides tailored expertise to investigators to assist in the design of molecular analyses and data interpretation, to optimize use of valuable biological samples and resources. The facility will be managed by a cadre of specialists with strong complementary expertise in molecular analysis, research facility administration, training, and mentoring of junior faculty. The Core equipment is primarily housed in dedicated space in close proximity to most users. This centralization of resources and knowledge provides consolidated and cost-effective analytical services and technical expertise to the Center members and users. The Core is organized in three units: Metals Analytical Chemistry, Oxidative Stress, and Biological Mass Spectrometry. The Specific Aims of this Core are: 1) to create a centralized, state-of-the-art resource that provides expertise, training and instrumentation for integrative molecular analysis, focused on metal interactions with biological systems; 2) to support the research objectives of mentored PIs and cultivate a new cohort of COBRE investigators within the Center for Metals in Biology and Medicine; and 3) develop a user base of established, funded investigators for continued growth and sustainability of the core facility. The expected outcomes include enhanced multidisciplinary research capabilities that support metals programs of mentored PIs in their trajectory toward R01 level funding, and increased collaborations within UNM, and across New Mexico and other IDeA states. These outcomes will positively impact the level of research excellence at UNM and enhance investigations into novel therapeutics for metal-mediated diseases that afflict millions of people in New Mexico and worldwide.