TIME RESOLVED MEASUREMENTS OF HEMOGLOBIN & MYOGLOBIN UNDER HYPERBARIC CONDITIONS

了解血红蛋白解离和释放的机制 缔合是研究血红蛋白稳定性和稳定性的基本因素 生产以血红蛋白为基础的胞外氧气载体。 不同交联物和修饰血红蛋白的临床研究 狂欢的症状是由于快速的肾脏过滤和 加速自氧化。我们正在利用超快的时间分辨 内源性色氨酸荧光法监测血红蛋白 在高静水压下解离成二聚体和单体 压力。我们的研究包括天然人类血红蛋白以及 人和牛的血红蛋白发生了交联化和突变。

Understanding the mechanism of hemoglobin dissociation and association is a fundamental factor to study hemoglobin stability and production of hemoglobin based extracellular oxygen carriers. Clinical studies with different crosslinked and modified hemoglobins reveled symptoms which are due to fast kidney filtration and accelerated autoxidation. We are utilizing ultrafast time-resolved intrinsic tryptophan fluorescence for monitoring the hemoglobin dissociation into the dimers and monomers under high hydrostatic pressure. Our study include native human hemoglobin as well as crosslinked and mutated human and bovine hemoglobins.