TIME RESOLVED MEASUREMENTS OF HEMOGLOBIN & MYOGLOBIN UNDER HYPERBARIC CONDITIONS

了解血红蛋白解离的机制， 缔合是研究血红蛋白稳定性的基本因素， 基于血红蛋白的细胞外氧载体的产生。 不同交联和修饰血红蛋白的临床研究 揭示的症状是由于快速肾过滤和 加速自氧化 我们利用超快时间分辨 用于监测血红蛋白的固有色氨酸荧光 在高静水压下解离成二聚体和单体 压力 我们的研究包括天然人血红蛋白以及 交联和突变的人和牛血红蛋白。

Understanding the mechanism of hemoglobin dissociation and association is a fundamental factor to study hemoglobin stability and production of hemoglobin based extracellular oxygen carriers. Clinical studies with different crosslinked and modified hemoglobins reveled symptoms which are due to fast kidney filtration and accelerated autoxidation. We are utilizing ultrafast time-resolved intrinsic tryptophan fluorescence for monitoring the hemoglobin dissociation into the dimers and monomers under high hydrostatic pressure. Our study include native human hemoglobin as well as crosslinked and mutated human and bovine hemoglobins.