TIME RESOLVED MEASUREMENTS OF HEMOGLOBIN & MYOGLOBIN UNDER HYPERBARIC CONDITIONS

血红蛋白的时间分辨测量

• 批准号: 6444734
• 负责人: ENRICO BUCCI
• 金额: $ 29.31万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444734
• 关键词: biological products; biomedical resource; biotechnology; hematology; informatics; spectrometry

Understanding the mechanism of hemoglobin dissociation and association is a fundamental factor to study hemoglobin stability and production of hemoglobin based extracellular oxygen carriers. Clinical studies with different crosslinked and modified hemoglobins reveled symptoms which are due to fast kidney filtration and accelerated autoxidation. We are utilizing ultrafast time-resolved intrinsic tryptophan fluorescence for monitoring the hemoglobin dissociation into the dimers and monomers under high hydrostatic pressure. Our study include native human hemoglobin as well as crosslinked and mutated human and bovine hemoglobins.

了解血红蛋白解离机制和 关联是研究血红蛋白稳定性的基本因素 产生基于血红蛋白的细胞外氧载体。 不同交联和修饰血红蛋白的临床研究 由于肾脏快速过滤而出现的症状 加速自氧化。 我们正在利用超快时间分辨 用于监测血红蛋白的内在色氨酸荧光 在高静水压下解离成二聚体和单体 压力。 我们的研究包括天然人类血红蛋白以及 交联和突变的人类和牛血红蛋白。