EFFECTS OF ULTRASONIC RADIATION ON FETAL GONADS

超声波辐射对胎儿性腺的影响

• 批准号: 3320716
• 负责人: FLOYD DUNN
• 金额: $ 12.7万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3320716
• 关键词: diagnosis design /evaluation; diagnosis procedure safety; embryo /fetus; gonads; histopathology; laboratory mouse; ultrasonography; ultrasound biological effect; ultrasound therapy; whole body irradiation effect

The clinical use of ultrasound for examination of the fetus has been available for nearly three decades. Because no deleterious effects have been detected in the exposed population, lack of risk has been assumed. However, the existence of subtle effects cannot be precluded. The initial study, "Study of Effects of Ultrasound Radiation of Fetal Gonads," was undertaken to determine if such effects occur in the fetal gonad following in utero exposure to 1 MHz ultrasound. Results of this study showed that adverse effects occurred in the fetal testis subjected to doses of ultrasound which have clinical relevance. These effects have the potential to cause reduced fertility or sterility in the adult animal. It is the purpose of this proposed study to determine the extend of these effects in the adult animal and to assess the role of heat and the resulting stress in the physical mechanism of interaction. To assess effects in the adult, experimental animals will receive in utero ultrasound exposure, be raised to maturity, and the testes subjected to extensive morphometric and histopathological analyses. Findings will be compared to data from sham and cage control specimens. The endpoints analyzed will give specific information regarding the aspect of testicular development which has been altered. The mechanism of heat will be investigated by quantifying histopathological data from testes of fetal mice heated by ultrasonic absorption and heated by a completely different thermal source. The contribution of stress as determined by increased corticosterone concentrations in the dam will also be assessed as imbalance in this hormone in fetal life is known to cause severe reproductive consequences in the adult male. To determine this, quantitative histopathological data from fetuses whose mothers received injections of exogenous corticosterone will be compared to those whose mothers received in utero ultrasound exposure. Experiments will also be carried out to determine the fetal effects of 3.5 MHz focussed ultrasound and if such observed effects are passed on to progeny. The results of this study will provide further understanding of the interaction of ultrasound and the fetal testis as well as provide a basis of risk assessment to the adult who has received in utero ultrasound exposure.

超声波在胎儿检查中的临床应用已被广泛应用。 可供使用近30年。因为没有有害的影响 如果在暴露人群中检测到该病毒，则假定该病毒缺乏风险。 然而，不能排除存在微妙的影响。这个 超声辐射对胎儿影响的初步研究 性腺，“是为了确定这种影响是否发生在胎儿 宫内暴露在1兆赫超声波下的性腺。这样做的结果是 研究表明，受试者的胎儿睾丸会出现不良反应 对于具有临床相关性的超声波剂量。这些影响有 导致成人生育能力下降或不育的可能性 动物。这项拟议的研究的目的是确定 在成年动物中的这些影响，并评估高温和 由此产生的应力在物理机制上相互作用。评估 在成年后，实验动物在子宫中会受到影响 超声波照射，被提高到成熟，并对睾丸进行 广泛的形态计量学和组织病理学分析。调查结果将是 与来自假标本和笼子对照标本的数据进行比较。终端 分析将提供有关以下方面的具体信息 睾丸发育已发生改变。热的作用机理 将通过量化来自睾丸的组织病理学数据进行研究 用超声吸收加热胎鼠和完全用超声波加热 不同的热源。压力的贡献由以下因素决定 大坝中皮质酮浓度的增加也将得到评估。 因为已知胎儿生命中这种激素的不平衡会导致严重的 成年雄性的生殖后果。为了确定这一点， 其母亲接受手术的胎儿的定量组织病理学数据 注射外源性皮质酮将与注射外源性皮质酮 母亲在宫内接受超声照射。实验也将是 以确定3.5 MHz聚焦超声对胎儿的影响 如果这种观察到的影响传递给后代。结果是 这项研究将提供进一步的了解相互作用。 超声波和胎儿睾丸以及提供风险的基础 对已接受宫内超声照射的成人进行评估。