PLATELET STUDIES IN THROMBOCYTOPENIA

我们建议研究抗体与血小板的结合， 特发性血小板减少性紫癜、血小板减少性紫癜 高血小板结合IgG、药物诱导的血小板减少症和同种免疫 血小板减少症由于HLA和其他抗原。 我们建议向 确定血小板结构中的糖蛋白， 抗体通过放射免疫沉淀相互作用，交叉 免疫电泳和“Western”印迹电泳。 为了 为了更准确地对这些抗体所携带的蛋白质进行分类 相互作用，一组在小鼠中制备的单克隆抗体 杂交瘤和鉴定血小板的各种蛋白质的方法将被 进行了 与这些抗体反应的蛋白质将与 具有临床意义的抗体。 证明单克隆 抗体和具有临床意义的抗体与其反应 通过抑制研究和抗原去除获得蛋白质 问题研究 蛋白质的身份将通过电泳法确定。 功能测试的移动性。 IgG也可以通过Fc受体结合。 当IgG 当被分子的Fab部分结合时，Fc受体被占据。 我们建议确定这是否是由抗体的Fc部分 分子。 我们建议定量研究的关系 Fc受体的结合和释放之间的关系 血小板的反应。

We propose to investigate the binding of antibodies to platelets in patients with idiopathic thrombocytopenic purpura, thrombocytopenia with high platelet-bound IgG, drug-induced thrombocytopenia, and alloimmune thrombocytopenia due to HLA and other antigens. We propose to propose to identify the glycoproteins within the platelet structure with which these antibodies interact by radioimmunoprecipitation, crossed immuno-electrophoresis, and electrophoresis with "Western" blot. In order to classify more accurately the proteins with which these antibodies interact, a panel of monoclonal antibodies which are made in mouse hybridomas and which identify various proteins of the platelet will be made. The proteins with which these antibodies react will be compared to results with clinically significant antibodies. Proof that the monoclonal antibody and the clinically significant antibody react with the same protein will be obtained by inhibition studies and by antigen removal studies. The identity of the protein will be determined by electrophoretic mobility on functional tests. IgG may also bind by Fc receptors. When IgG is bound by the Fab portion of the molecule, the Fc receptor is occupied. We propose to determine whether this is by the Fc portion of the antibody molecule. We propose to investigate quantitatively the relationships between the binding of the Fc receptor and the generation of the release reaction of the platelet.