HEMOGLOBIN BASED OXYGEN CARRIERS

我们建议探索人和牛的血红蛋白作为 输液用氧气载体。其目的是为了获得高度的 氧气法纯化的两种血红蛋白的不解离四聚体 亲和力与体内氧转运相容。这项研究将 包括开发必要的化合物，对其进行全面分析 其理化和功能特性及其初步测试 在小型动物中的储存稳定性和保留时间。一步法化学 反应将用于获得所需的血红蛋白衍生物， 使用有限和特定的化学修饰，以避免形成 具有高抗原性的产品。已经得到的两个化合物 通过双二溴水杨基富马酸与牛血红蛋白的交联，将得到 广泛提纯和分析其功能特性和部位 交叉链接。将为人类血红蛋白设计新的治疗方法 交联物具有比所需的更高的氧亲和力 跨功能液体。

We propose to explore the potential of human and bovine hemoglobins as oxygen carriers for transfusional fluids. The aim is to obtain highly purified indissociable tetramers of the two hemoglobins with an oxygen affinity compatible with oxygen transport in vivo. The research will involve the development of the necessary compounds, full analyses of their physico-chemical and functional properties, and initial testing of their storage stability and retention time in small animals. One-step chemical reactions will be used for obtaining the desired hemoglobin derivatives, using limited and specific chemical modifications, so as to avoid formation of products with high antigenic activity. Two compounds already obtained by cross-linking bovine hemoglobin with bis-dibromosalicyl-fumarate will be extensively purified and analyzed for their functional properties and sites of cross-links. New treatments will be designed for human hemoglobin whose cross-linked products have an oxygen affinity higher than that desirable in transfunctional fluids.