HEMOGLOBIN-BASED OXYGEN CARRIERS--DESIGN AND APPLICATION

基于血红蛋白的载氧体——设计与应用

• 批准号: 2459981
• 负责人: ENRICO BUCCI
• 金额: $ 118.85万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2459981
• 关键词: blood /plasma substitute; hemoglobin; respiratory oxygen

The development and application in vivo of artificial oxygen carriers of known and controllable characteristics offers a new approach to the exploration of uptake, delivery and utilization of oxygen by the various tissues. We will focus our efforts on the production of artificial hemoglobin-based oxygen carriers with highly controlled physico-chemical and functional properties, and test their effects on specific animal models. It is a highly interdisciplinary effort which involves molecular simulations, crystallography, genetic engineering, chemical synthesis, renal function tests and brain physiology. The strategy of the program includes two main components: Molecular design and in vivo applications.

人工氧载体的开发及体内应用 已知且可控的特性提供了一种新的方法 探索各种生物体对氧气的吸收、输送和利用 组织。 我们将重点致力于人造板的生产 具有高度受控物理化学性质的基于血红蛋白的氧载体 和功能特性，并测试它们对特定动物的影响 模型。 这是一项高度跨学科的工作，涉及分子 模拟、晶体学、基因工程、化学合成、 肾功能检查和脑生理学。 计划的策略 包括两个主要部分：分子设计和体内应用。

项目成果

Cerebral blood flow during hypoxic hypoxia with plasma-based hemoglobin at reduced hematocrit.

缺氧时的脑血流量，血细胞比容降低时血浆血红蛋白。

• DOI: 10.1152/ajpheart.1998.274.6.h1933
• 发表时间: 1998
• 期刊: The American journal of physiology
• 作者: Ulatowski,JA;Bucci,E;Razynska,A;Traystman,RJ;Koehler,RC
• 通讯作者: Koehler,RC

Molecular engineering of a polymer of tetrameric hemoglobins.

四聚血红蛋白聚合物的分子工程。

• DOI: 10.1002/prot.1086
• 发表时间: 2001
• 期刊: Proteins.
• 作者: Fronticelli,C;Arosio,D;Bobofchak,KM;Vasquez,GB
• 通讯作者: Vasquez,GB

Renal response to hemodilution with albumin or crosslinked bovine hemoglobin: role of nitric oxide.

白蛋白或交联牛血红蛋白血液稀释的肾脏反应：一氧化氮的作用。

• DOI: 10.1016/s0022-2143(98)90024-5
• 发表时间: 1998
• 期刊: The Journal of laboratory and clinical medicine.
• 作者: Matheson,B;Razynska,A;O'Hearne,M;Bucci,E
• 通讯作者: Bucci,E

Tyrosine confounds oxidative electrochemical detection of nitric oxide.

酪氨酸混淆一氧化氮的氧化电化学检测。

• DOI: 10.1152/ajpheart.1998.274.5.h1698
• 发表时间: 1998
• 期刊: The American journal of physiology
• 作者: Stingele,R;Wilson,DA;Traystman,RJ;Hanley,DF
• 通讯作者: Hanley,DF

Positive and negative cooperativities at subsequent steps of oxygenation regulate the allosteric behavior of multistate sebacylhemoglobin.

随后的氧合步骤中的正向和负向协同作用调节多态癸二酰血红蛋白的变构行为。

• DOI: 10.1021/bi952446b
• 发表时间: 1996
• 期刊: Biochemistry.
• 作者: Bucci,E;Razynska,A;Kwansa,H;Gryczynski,Z;Collins,JH;Fronticelli,C;Unger,R;Braxenthaler,M;Moult,J;Ji,X;Gilliland,G
• 通讯作者: Gilliland,G