ORGANELLE MOBILITY IN LIVING CELLS

活细胞中的细胞器移动性

• 批准号: 3351461
• 负责人: PETER A VALBERG
• 金额: $ 14.59万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3351461
• 关键词: actins; aerosols; alkaloids; alveolar macrophages; biological models; biomagnetism; biomagnetism measurement; biotechnology; calcium transporting ATPase; cell biology; cell motility; cell population study; chemotaxis; cytochalasins; cytoplasm; cytoskeleton; electron microscopy; immunofluorescence technique; magnetic field; mathematical model; microtubules; particle; phagocytosis; photomicrography; protein kinase C; tissue /cell culture; vesicle /vacuole; video recording system; viscosity

Pulmonary macrophages defend the respiratory surfaces of the lungs against deposited particles through their phagoctic and migratory capabilities. a novel method, involving microscopic magnetic particles, promises to be a valuable expermental indicator of macrophage function, by providing information noninvasively on the motions of cell organelles. When humans or animals inhale a magnetic-particle aerosol, a fraction of the particles are retained in the lungs and ingested by pulmonary macrophages. We have found that for phagocytized particles, cytoplasmic motions rotate the particles and produce detectable changes in the magnetic field. Thus, sensitive magnetometry may serve as an experimental probe of the motile status of macrophages, specifically, a probe not rrequiring optical observation of cells on the stage of a microscope. In addition, intracellular particles can be twisted by an applied external field, which allows measurement of cytosol viscoelasticity. The proposed research will examine magnetometric phenomena for lung macrophages in culture. We week to answer the following question: (1) How do magnetometric signals correlate to optically-observed cell organelle motions? (2) How do magnetometrically-measured motion and viscoelasticity change with macrophage functional status and cytoskeletal integrity? (3) Can intracellular magnetic particle motions be used to help formulate and test models of intracellular force generation and viscoelasticity? The goal is to critically examine magnetometry as a non-optical method for probing cellular motile proceses. Magnetometry may provide a unique diagnostic procedure for in situ assessment of the behavior of phagocvytic cells.

肺巨噬细胞保卫肺脏呼吸面 肺通过吞噬和 迁徙能力。一种新的方法，涉及显微 磁性粒子有望成为一项有价值的实验 巨噬细胞功能的指示器，通过提供信息 非侵入性地影响细胞器的运动。 当人类或动物吸入磁性粒子气雾剂时， 一部分颗粒物留在肺部，并被 肺巨噬细胞。我们已经发现，对于吞噬 颗粒，细胞质的运动旋转颗粒并产生 磁场中可检测到的变化。因此，敏感 磁学可以作为运动学的一种实验探头。 巨噬细胞的状态，具体地说，不需要光学的探针 在显微镜的舞台上观察细胞。此外, 细胞内的颗粒可以被施加的外场扭曲， 这使得可以测量胞浆粘弹性。 这项拟议的研究将检查磁学现象。 用于肺巨噬细胞的培养。我们将在本周回答以下问题 问题： (1)磁测信号如何与光学观测相关？ 细胞器运动？ (2)磁力测量的运动和 粘弹性随巨噬细胞功能状态的变化 细胞骨架的完整性？ (3)细胞内的磁粒子运动能否提供帮助？ 建立和测试细胞内力产生和测试模型 粘弹性？ 其目标是将磁学作为一种非光学方法进行批判性检查 探测细胞运动过程的方法。磁学测量可以 提供一种独特的诊断程序，用于现场评估 吞噬细胞的行为。