MAGNETIC FIELD EFFECTS ON MACROPHAGES

人体组织不是铁磁性的，而是铁磁性颗粒 可以作为污染物存在于肺部和其他器官中。 吸入肺部的空气中的磁性颗粒 沉积在肺表面并被肺摄取 巨噬细胞 这些细胞保护呼吸道表面， 通过它们的吞噬和迁移能力。 强磁场使磁性粒子移动， 磁性对齐。 这可能会损害 含有铁磁性颗粒的巨噬细胞。 细胞内的微观磁性颗粒也可以用作 细胞功能的探针 我们把磁性粒子 吸入气雾剂，并测量磁场， 通过肺灌洗从暴露动物中回收巨噬细胞。 这些粒子是微小的永久磁铁，当它们排列在一起时， 它们会产生一个可以测量的反射场 用灵敏的磁力计非侵入性地测量 当 粒子移动和旋转，反射场改变。 因此 对于肺巨噬细胞摄入的颗粒，细胞质 运动使粒子旋转远离对齐方向 并导致反射场减小。 此外，本发明还提供了一种方法， 细胞内颗粒可以被外场扭曲， 它们旋转的程度是衡量 细胞质粘度 这项研究将系统地研究磁 现象在培养的巨噬细胞。 其目标是：1） 校准磁力测量技术， 巨噬细胞功能状态。 2)制定各种程序来 提取有意义的参数 剩余磁场曲线 3)使用磁信号作为 一种用于细胞质运动和细胞质流变学的探针。 四、 测量产生的磁信号的变化， 巨噬细胞暴露于强外部磁场。 这一结果将有助于建立细胞内磁场的测量方法。 铁磁颗粒作为一种非光学方法， 监测细胞内的生物过程， 磁场的影响。 因为磁性粒子 在完整的动物和人类中也可以检测到， 磁力测定法最终可以作为非侵入性的 原位巨噬细胞功能的探针。

Body tissues are not ferromagnetic, but ferromagnetic particles can be present as contaminants in the lungs and other organs. Airborne magnetic particles that are inhaled into the lungs become deposited on lung surfaces and are ingested by lung macrophages. These cells defend the respiratory surfaces of the lungs through their phagocytic and migratory capabilities. Strong magnetic fields cause magnetic particles to move and magnetically align. This may be detrimental the function of macrophages containing ferromagnetic particles. Microscopic magnetic particles within cells can also be used as a probe of cell function. We delivered magnetic particles by aerosol inhalation and measured the magnetic fields from macrophages receovered by lung lavage from exposed animals. The particles are tiny permanent magnets, and when aligned, they produce a remanent field which can be measured non-invasively with a sensitive magnetometer. When the particles move and rotate, the remanent field changes. Thus for particles ingested by pulmonary macrophages, cytoplasmic motions rotate particles away from the direction of alignment and cause the remanent field to decrease. In addition, intracellular particles can be twisted by an external field, and the degree to which they rotate in response is a measure of cytosol viscosity. The research proposed will systematically investigate magnetic phenomena in cultured macrophages. The aims will be to: 1) Calibrate the magnetometric technique as a measure of macrophage functional status. 2) Establish procedures for extracting meaningful parameters from the remanent-magnetic-field curves. 3) Use the magnetic signals as a probe for cytoplasmic motion and cytosol rhealogy. 4) Measure the changes in magnetic signals generated when macrophages are exposed to strong external magnetic fields. The results will help establish magnetometry of intracellular ferromagnetic particles as a non-optical method both for monitoring biologic processes within cells and for determining the effects of magnetic fields. Since magnetic particles within intact animals and humans can also be detected, magnetometry may ultimately find application as a non-invasive probe of in situ macrophage function.