MAGNETIC FIELD EFFECTS ON MACROPHAGES

磁场对巨噬细胞的影响

• 批准号: 3180993
• 负责人: PETER A VALBERG
• 金额: $ 15.34万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-12-01 至 1988-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3180993
• 关键词: aerosols; alveolar macrophages; cell motility; cytoplasm; diagnostic respiratory lavage; hamsters; iron; macrophage; magnetic field; magnetism; mathematical model; nonblood rheology; particle; phagocytosis; tissue /cell culture; video recording system; viscosity

Body tissues are not ferromagnetic, but ferromagnetic particles can be present as contaminants in the lungs and other organs. Airborne magnetic particles that are inhaled into the lungs become deposited on lung surfaces and are ingested by lung macrophages. These cells defend the respiratory surfaces of the lungs through their phagocytic and migratory capabilities. Strong magnetic fields cause magnetic particles to move and magnetically align. This may be detrimental the function of macrophages containing ferromagnetic particles. Microscopic magnetic particles within cells can also be used as a probe of cell function. We delivered magnetic particles by aerosol inhalation and measured the magnetic fields from macrophages receovered by lung lavage from exposed animals. The particles are tiny permanent magnets, and when aligned, they produce a remanent field which can be measured non-invasively with a sensitive magnetometer. When the particles move and rotate, the remanent field changes. Thus for particles ingested by pulmonary macrophages, cytoplasmic motions rotate particles away from the direction of alignment and cause the remanent field to decrease. In addition, intracellular particles can be twisted by an external field, and the degree to which they rotate in response is a measure of cytosol viscosity. The research proposed will systematically investigate magnetic phenomena in cultured macrophages. The aims will be to: 1) Calibrate the magnetometric technique as a measure of macrophage functional status. 2) Establish procedures for extracting meaningful parameters from the remanent-magnetic-field curves. 3) Use the magnetic signals as a probe for cytoplasmic motion and cytosol rhealogy. 4) Measure the changes in magnetic signals generated when macrophages are exposed to strong external magnetic fields. The results will help establish magnetometry of intracellular ferromagnetic particles as a non-optical method both for monitoring biologic processes within cells and for determining the effects of magnetic fields. Since magnetic particles within intact animals and humans can also be detected, magnetometry may ultimately find application as a non-invasive probe of in situ macrophage function.

人体组织不是铁磁性的，而是铁磁性的颗粒 可能以污染物的形式存在于肺部和其他器官。 吸入肺部的空气中的磁性粒子 沉积在肺表面，并被肺摄取 巨噬细胞。这些细胞保卫人类的呼吸表面 肺通过其吞噬和迁移能力。 强磁场使磁性粒子移动并 磁力对准。这可能有损于 含有铁磁性颗粒的巨噬细胞。 细胞内的微小磁性颗粒也可以用作 细胞功能的探测仪。我们通过以下方式运送磁性颗粒 气雾剂吸入并测量了来自 通过肺灌洗从暴露的动物体内恢复巨噬细胞。 这些粒子是微小的永久磁铁，当它们排列在一起时， 它们会产生一个可以测量的剩余场 使用灵敏的磁力计进行非侵入性测量。当 粒子运动和旋转时，剩余场发生变化。因此， 对于肺巨噬细胞摄取的颗粒，胞浆 运动使粒子远离对齐方向 并导致剩余场减小。此外, 细胞内的颗粒可以被外场扭曲， 它们的旋转反应程度是衡量 胞浆粘度。 提出的研究将系统地研究磁力 巨噬细胞培养中的现象。目标将是：1) 校准磁力测量技术作为测量 巨噬细胞功能状态。2)建立以下程序： 中提取有意义的参数。 剩余磁场曲线。3)使用磁信号作为 细胞质运动和胞浆流变学的探针。4) 测量在以下情况下产生的磁信号的变化 巨噬细胞暴露在强大的外部磁场中。 这一结果将有助于建立细胞内的磁测量 铁磁性颗粒作为一种非光学方法 监测细胞内的生物过程并确定 磁场的影响。由于磁性颗粒 在完好无损的动物和人类体内也可以检测到， 磁测量最终可能会作为一种非侵入性技术得到应用 原位巨噬细胞功能的探查。