ORGANELLE MOBILITY IN LIVING CELLS

活细胞中的细胞器移动性

• 批准号: 3351460
• 负责人: PETER A VALBERG
• 金额: $ 16.34万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3351460
• 关键词: actins; aerosols; alkaloids; alveolar macrophages; biological models; biomagnetism; biomagnetism measurement; biotechnology; calcium transporting ATPase; cell biology; cell motility; cell population study; chemotaxis; cytochalasins; cytoplasm; cytoskeleton; electron microscopy; immunofluorescence technique; magnetic field; mathematical model; microtubules; particle; phagocytosis; photomicrography; protein kinase C; tissue /cell culture; vesicle /vacuole; video recording system; viscosity

Pulmonary macrophages defend the respiratory surfaces of the lungs against deposited particles through their phagoctic and migratory capabilities. a novel method, involving microscopic magnetic particles, promises to be a valuable expermental indicator of macrophage function, by providing information noninvasively on the motions of cell organelles. When humans or animals inhale a magnetic-particle aerosol, a fraction of the particles are retained in the lungs and ingested by pulmonary macrophages. We have found that for phagocytized particles, cytoplasmic motions rotate the particles and produce detectable changes in the magnetic field. Thus, sensitive magnetometry may serve as an experimental probe of the motile status of macrophages, specifically, a probe not rrequiring optical observation of cells on the stage of a microscope. In addition, intracellular particles can be twisted by an applied external field, which allows measurement of cytosol viscoelasticity. The proposed research will examine magnetometric phenomena for lung macrophages in culture. We week to answer the following question: (1) How do magnetometric signals correlate to optically-observed cell organelle motions? (2) How do magnetometrically-measured motion and viscoelasticity change with macrophage functional status and cytoskeletal integrity? (3) Can intracellular magnetic particle motions be used to help formulate and test models of intracellular force generation and viscoelasticity? The goal is to critically examine magnetometry as a non-optical method for probing cellular motile proceses. Magnetometry may provide a unique diagnostic procedure for in situ assessment of the behavior of phagocvytic cells.

肺巨噬细胞保护肺的呼吸表面， 肺通过其吞噬和 迁移能力。 一种新的方法， 磁性粒子，有望成为一个有价值的实验， 通过提供信息来指示巨噬细胞功能 非侵入性地影响细胞器的运动。 当人类或动物吸入磁性颗粒气溶胶时， 部分颗粒保留在肺中并被吸入 肺巨噬细胞 我们发现，对于被吞噬的 细胞质运动使颗粒旋转， 磁场中可检测到的变化。 因此，敏感 磁力测量可以作为运动物体的实验探针 巨噬细胞的状态，特别是，不需要光学的探针 在显微镜的载物台上观察细胞。 此外，本发明还提供了一种方法， 细胞内颗粒可以被施加的外部场扭曲， 其允许测量胞质溶胶粘弹性。 拟议的研究将检查磁力现象 培养的肺巨噬细胞。 我们一周来回答以下问题 问： (1)磁力信号如何与光学观测的 细胞器运动 (2)磁力测量的运动和 黏弹性随巨噬细胞功能状态的变化， 细胞骨架完整性 (3)细胞内的磁性粒子运动能帮助 制定和测试细胞内力产生的模型， 粘弹性？ 我们的目标是严格审查磁力作为一个非光学 探测细胞运动过程的方法。 磁力测量可以 提供了一个独特的诊断程序，用于原位评估 吞噬细胞的行为。