MAGNETIC FIELD EFFECTS ON MACROPHAGES

磁场对巨噬细胞的影响

• 批准号: 3180994
• 负责人: PETER A VALBERG
• 金额: $ 15.2万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3180994
• 关键词: aerosols; alveolar macrophages; cell migration; cell motility; chemotaxis; cytoplasm; diagnostic respiratory lavage; hamsters; intracellular parasitism; iron oxide; lysosomes; magnetic field; magnetic resonance imaging; magnetism; membrane fusion; nonblood rheology; particle; phagocytosis; photomicrography; tissue /cell culture; vesicle /vacuole; video recording system; viscosity

In vivo nuclear magnetic resonance (NMR) exposes living tissue to large magnetic fields. Tissue constituents interact weakly with magnetic fields, and the direct effects of magnetic fields on cellular, biochemical, and genetic processes have not been reproducibly established. However, microscopic ferromagnetic particles be present as contaminants in the lungs and other organs. in many occupational settings, humans inhale magnetic-particle aerosols; a fraction of the particles are retained in the lungs and are ingested by pulmonary macrophages. Strong magnetic fields can cause these magnetic particles to move, rotate, and agglomerate. The toxicology of NMR fields on cells and tissues containing ferromagnetic particles is unknown. Years 01 and 02 of the project demonstrated that microscopic magnetic particles can be observed both optically and magnetically in cells. Moreover, such particles can serve as an experimental indicator of macrophage function by noninvasively providing information on the motions of cell organelles. We found that for phagocytized particles, cytoplasmic motions rotate particles and produce detectable changes in the magnetic field. In addition, intracellular particles can be twisted by an applied external field, which allows measurement of cytosol viscoelasticity. The proposed researeh will examine magnetie field effects on particle-containing lung macrophages. In years 04-06 we will address the following questions: (1) Can magnetic forces overcome the motile forces generated by the macrophage cytoplasm? Can magnetic forces restrain chemotaxis? We will quantify the magnitude of traction forces magnetometrically. (2) Can motion imposed by magnet fields on magnetic-particle containing cell organelles modulate the fusion of phagocytic and lysosomal vesicles? (3) Can alterations in cell organelle motions, magnetometrically detected, be used to identify the existence and activity of an intracellular infection (e.g., AIDS) in macrophages? The goal is to examine how phagocytized ferromagnetic particles modify cell function when cells are exposed to strong magnetic fields. At the same time, we will measure the forces generated in cell motility, we will examine the role of organelle motions in phagolysosome processing, and we will identify changes in motion and rheology caused by viral infection.

体内核磁共振（NMR）将活组织暴露于 大磁场。 组织成分与 磁场，以及磁场对 细胞、生物化学和遗传过程还没有被 可重复建立。 然而，微观铁磁性 颗粒可能作为污染物存在于肺和其他器官中。 在许多职业环境中，人类吸入磁性粒子， 气溶胶;一部分颗粒保留在肺部， 被肺巨噬细胞摄取 强磁场可以 导致这些磁性粒子移动、旋转和聚集。 核磁共振场对细胞和组织的毒理学研究 铁磁粒子是未知的。 01年和02年的项目表明，微观 磁性粒子可以用光学和磁性两种方法观察到 在细胞中。 此外，这种粒子可以作为实验 通过非侵入性提供巨噬细胞功能指示剂 细胞器运动的信息。 我们发现， 吞噬颗粒，细胞质运动旋转颗粒， 在磁场中产生可检测的变化。 此外，本发明还提供了一种方法， 细胞内颗粒可以被施加的外部 场，其允许测量胞质溶胶粘弹性。 拟议的研究将检查磁场对 含颗粒的肺巨噬细胞。 04-06年， 回答以下问题： (1)磁力能否克服由 巨噬细胞的细胞质 磁力能抑制趋化性吗？ 我们将量化牵引力的大小 用磁力计 (2)磁场对磁性粒子的作用 含有细胞器调节吞噬细胞和 溶酶体囊泡 (3)细胞器运动的改变， 检测，用于识别存在和活动的 细胞内感染（例如，艾滋病）在巨噬细胞？ 目的是研究吞噬的铁磁颗粒 当细胞暴露于强磁场时， 领域的 与此同时，我们将测量 细胞运动，我们将研究细胞器运动的作用， 吞噬溶酶体处理，我们将识别运动的变化， 以及由病毒感染引起的流变性。