COMPARISON OF OPIATE/OPIOID RECEPTORS IN THE ANS AND CNS
COMPARISON OF OPIATE/OPIOID RECEPTORS IN THE ANS AND CNS
批准号:
3398046
负责人:
JAY A GLASEL
金额:
$7.41万
依托单位国家:
美国
项目类别:
财政年份:
1983
资助国家:
美国
项目状态:
已结题
起止时间:
1983-01-01 至 1986-06-30
中文摘要
这是重新提交的1R01 NS18017-01,已被批准
英文摘要
This is a resubmission of 1R01 NS18017-01 which was approved with good
priority but not funded. The proposal has been revised and updated in the
light of further preliminary work and study section suggestions. The basic
objective of the research is the isolation and characterization, at the
molecular level, of opiate and opioid receptors derived from subcellular
fractions of CNS and ANS neural tissue. The current evidence for the
existence of multiple opiate/opioid receptors in CNS and ANS tissue demands
clarification and is closely allied with integrative phenomena in both
nervous systems. We will first concentrate on a programmed use of
radiation inactivation techniques to determine the gross molecular features
of receptors in the different tissues. The step beyond this will be to
raise, purify, isolate and characterize monoclonal anti-receptor
antibodies. These will be used as tools for investigating receptor
heterogeneity in tissue situated receptors in more detail. They will also
serve as starting points for more detailed in vivo physiological work to be
carried out beyond this grant period. We will also use these materials as
affinity purification reagents in the isolation of receptor
macromolecules. In addition, we will collaborate with at least one other
laboratory on the development of new ligands for opiate/opioid receptors
which would aid us in obtaining more complete and reliable characterization
of their properties. In particular we have in mind possible development of
ligands which may have selective interaction profiles towards Mu, Delta or
Kappa receptors. Our long range goal is understanding the sequence of
molecular events in neuromembranes following opiate/opioid ligand reception
and their integration into the CNS (as exemplified by spino-thalamic, and
spino-reticulo thalamic pain pathways) and ANS (as exemplified by the
relationships between visceral-sensory and visceral-motor pathways).
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
BIO-AND IMMUNO-CHEMICAL STUDIES ON OPIATE RECEPTORS
-
批准号:3211717
-
项目类别:
-
资助金额:$11.17万
-
财政年份:1988
-
负责人:JAY A GLASEL
-
依托单位:
BIO-AND IMMUNO-CHEMICAL STUDIES ON OPIATE RECEPTORS
-
批准号:3211718
-
项目类别:
-
资助金额:$11.73万
-
财政年份:1988
-
负责人:JAY A GLASEL
-
依托单位:
BIO-AND IMMUNO-CHEMICAL STUDIES ON OPIATE RECEPTORS
-
批准号:3211714
-
项目类别:
-
资助金额:$9.55万
-
财政年份:1988
-
负责人:JAY A GLASEL
-
依托单位:
CONFORMATIONS OF LIGANDS AT ANTIBODY BINDING SITES
-
批准号:3396426
-
项目类别:
-
资助金额:$6.6万
-
财政年份:1979
-
负责人:JAY A GLASEL
-
依托单位:
CONFORMATIONS OF LIGANDS AT ANTIBODY BINDING SITES
-
批准号:3396425
-
项目类别:
-
资助金额:$9.46万
-
财政年份:1979
-
负责人:JAY A GLASEL
-
依托单位:
海外基金