DOPAMINERGIC NEURONS: CELL INTERACTIONS AND PLASTICITY

特定神经元寿命的改变可能会导致严重的 成熟生物体的行为缺陷。在这里提出的调查 将研究位置和细胞的变化 相互作用将影响多巴胺能神经元的存活。 免疫细胞化学和组织化学方法用于鉴定 多巴胺能神经元和胆碱能神经元。实验摄动 多巴胺能神经元系统的损伤将通过新生儿病变来进行 和移植技术。发育中脑多巴胺能 神经元将在原位部分去传入或移植到不同的 新生大鼠的大脑区域。最初，这些研究将测试 环境条件变化的影响(例如，不同的宿主大脑 区域、传入纤维类型的差异)。 多巴胺能神经元的可塑性及其对寿命的影响 神经元。定性和定量分析将在以下方面进行 光镜和电子显微镜水平。根据上述程序， 细胞间相互作用蛋白在多巴胺能神经元中的作用 胆碱能细胞(纹状体、顶盖)将在联合移植和 重新聚集移植物。因为突触是形态上的实体 表示神经元的相互作用，特别强调观察 在精细的结构层面上。

Alterations in longevity of specific neurons may bring about severe behavioral deficits of a mature organism. The investigations proposed here will study the question of whether changes in position and cellular interactions will affect survival of dopaminergic neurons. Immunocytochemical and histochemical approaches are to be used to identify dopaminergic and putative cholinergic neurons. Experimental perturbation of the dopaminergic neuron systems will be carried out by neonatal lesions and transplantation techniques. Developing mesencephalic dopaminergic neurons will be partially deafferented in situ or transplanted to various regions of the brain of newborn rats. Initally these studies will test the influence of variable environmental conditions (e.g. different host brain areas, differences in the types of afferent fibers) on the connection plasticity of dopaminergic neurons and their effect on longevity of such neurons. Qualitative and quantitative analysis will be carried out at light and electron microscopic levels. Following the above procedures, the effect of cell interactins between dopaminergic neurons and putative cholinergic cells (striatal, tectal) will be investigated in co-grafts and reaggregation grafts. Since synapses are the morphological entities that signify neuron interactions, particular emphasis is placed on observations at the fine structural level.