Generation of Aptamer-Molecularly Imprinted Polymer Hybrid Materials

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Molecular recognition (the ability to specifically recognise a chemical compound) is a highly important feature of analytical science. More often than not compound specific recognition comes from biological element such as enzymes, antibodies and DNA. Aptamers are short strands of DNA. Whilst the properties in terms of recognition for biological molecules are excellent, the performance and environmental stability is low and this can compromise their uses. In particular degradation is a major problem. To combat these derogatory issues a new generation of artifical recognition materials have been developed. At the forefront is a technology called Molecular Imprinting. This involves making a small binding pocket in a polymer which is chemically and shape specific for the target compound. These "smart plastics" offer the robustness and the ability to work in extreme environmental conditions but can lack the needed specificity/affinity.This project aims to create a "best of both worlds" scenario. By slightly changing the chemical structure of the aptamer DNA we intend to use the aptamer as the recognition part of a molecularly imprinted polymer (MIP). This will be achieved by making the aptamer polymerisable so it can become part of the polymer structure, incorporating it into the polymer matrix via polymerisable groups on the DNA.In this way we intend to preserve the high affinity and specificity of the aptamer whilst imparting the robustness and added shape specificity generated by the MIP. The presence of the polymer should protect the aptamer from environmental degradation and potentially widen the scope of use of aptamers for recognition.