DROSOPHILA NEURAL DEVELOPMENT ROLE OF BIOGENIC AMINES

这项建议涉及到关于发展分析的实验 生物胺系统及其在神经发育中的总体作用。 在生物胺的情况下，异常的神经递质水平 与许多人类病理有关的观察(例如 帕金森病、苯丙酮脲、精神分裂症)。在果蝇中 黑素胃镜，中枢神经系统(CNS)神经缺陷，解剖 来自缺乏多巴脱羧酶基因的动物 因此，已观察到内源性5-羟色胺和多巴胺缺乏。 具体而言，拟议研究的目的是：1)进一步 多巴脱羧酶的神经解剖学和生化特性 缺陷型和野生型CNS；2)发育的测定 观察到的扰动的原因；以及3)对 神经缺陷。为了解决这些问题，实验策略 依赖于基因工具、突变体和基因的复杂使用 将使用果蝇系统独有的马赛克动物。在……里面 此外，目前可用于可视化的常规工具 将使用生物胺系统和表征神经元组织。 这些工具包括针对神经递质和 神经肽，儿茶酚胺组织荧光，放射性胺， 免疫组织化学和放射自显影。该方法将有一个 强大的神经解剖学成分。预计在中国的快速发展 分子技术将提供额外的工具，进一步 提高对神经递质作用的因果分析的分辨率 在神经系统的发育和维护方面。

This proposal involves experiments concerning the analysis of development of biogenic amine systems and their overall role in neural development. Aberrant neurotransmitter levels in the case of biogenic amines have been observed in association with a number of human pathologies (e.g. Parkinson's disease, phenylketonurea, schizophrenia). In Drosophila melanogaster, neural defects in the central nervous system (CNS), dissected from animals genetically deficient for the enzyme dopa decarboxylase and therefore, lacking endogenous serotonin and dopamine have been observed. Specifically, the aims of the proposed research are: 1) further neuroanatomical and biochemical characterization of the dopa decarboxylase deficient and the wild-type CNSs; 2) determination of the developmental etiology of the observed perturbations; and 3) causal analyses of the neural defects. To approach these questions, experimental strategies that depend on the sophisticated use of genetic tools, mutants and genetically mosaic animals, unique to the Drosophila system, will be used. In addition, conventional tools that are currently available to visualize biogenic amine systems and characterize neuronal tissues will be employed. These tools include antibodies raised against neurotransmitters and neuropeptides, catecholamine histofluorescence, radioactive amines, immunohistochemistry and autoradiography. The methodology will have a strong neuroanatomical component. It is anticipated that rapid advances in molecular techniques will provide additional tools that will further sharpen the resolution of causal analysis of the role of neurotransmitters in the development and the maintenance of the nervous system.