NEUROBIOLOGY OF IDENTIFIED PEPTIDERGIC NEURONS

已鉴定的肽能神经元的神经生物学

• 批准号: 3409359
• 负责人: Nathan Tublitz
• 金额: $ 12.34万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3409359
• 关键词: biological models; chromatography; developmental neurobiology; electrophysiology; histology; neural information processing; neuroanatomy; neurochemistry; neuroendocrine system; neuropeptides; neurophysiology; nonmammalian vertebrate embryology

The long-term goal of this project is to understand at the cellular level the mechanisms which control the interactions between peptidergic neurons and other cells. Neuropeptides have profound effects on many body tissues including the nervous system of most animals from lower invertebrates to humans. They perform a wide variety of physiological roles ranging from neurotransmitter to neurohormone. The nervous system of the moth, Manduca sexta, provides a particularly attractive model system in which electrophysiological, endocrinological, biochemical and developmental experiments can be performed conveniently in vivo and in vitro on individually identifiable peptidergic neurons. The cellular action of neuropeptides can therefore be followed with greater precision in this invertebrate system than in any present vertebrate preparation, where cellular studies on neuropeptides have proven difficult. The primary focus of this study will be 6 individually identified peptidergic neurons in the moth which contain two novel neuropeptides. We have previously shown that these two peptides, known as cardioacceleratory peptides (CAPs), play important cardioregulatory roles during adult emergence behavior in the moth. This proposal will utilize a multidisciplinary approach to examine electrophysiological and biochemical aspects of CAP function. Little is known about the electrophysiological mechanisms that regulate the activity of peptide-containing neurons. Intracellular recordings from active CAP neurons will be obtained during adult emergence behavior. In these experiments, electrical activity in individual CAP neurons will be directly related to the amount of peptide released. Experiment, in which the neuronal inputs to the CAP neurons are identified, will provide crucial new information on the neural control of peptidergic neurons and peptide release. The mode of action of the CAPs on their targets will also be explored. The electrophysiological effects of the CAPs will be studied in individually dissociated heart muscle fibers using conventional and voltage clamp recordings. The biochemical basis of CAP action on the heart will also be investigated by measuring CAP induced changes in the intracellular levels of cyclic nucleotides and/or inositol phosphates.

本项目的长期目标是了解 在细胞水平上控制相互作用的机制 肽能神经元和其他细胞之间的联系。 神经肽 对许多身体组织有深远的影响， 从低级无脊椎动物到人类的大多数动物的系统。 它们发挥着各种各样的生理作用， 神经递质到神经激素。 的神经系统 天蛾，天蛾，提供了一个特别有吸引力的模型， 系统中，电生理，内分泌， 可以进行生化和发育实验， 方便地在体内和体外对个体可识别的 肽能神经元 神经肽的细胞作用可以 因此，在这种无脊椎动物中， 系统比在任何现有脊椎动物制剂中，其中 对神经肽的细胞研究已经证明是困难的。 本研究的主要重点将是6个单独确定的 肽能神经元在蛾，其中含有两个新的 神经肽 我们之前已经证明，这两个 肽，称为心脏加速肽（CAPs）， 在成人出现期间重要的心脏调节作用 蛾的行为 该提案将利用 多学科的方法来检查电生理和 CAP功能的生化方面。 人们对这种电生理机制知之甚少， 调节含肽神经元的活性。 来自活跃CAP神经元的细胞内记录将被 在成虫羽化过程中获得的。 在这些实验中， 单个CAP神经元的电活动将直接 与释放的肽的量有关。 实验，其中 识别到CAP神经元的神经元输入，将 提供了关于神经控制的重要新信息， 肽能神经元和肽释放。 联合呼吁程序对其目标的作用方式也将是 探讨了 CAP的电生理效应将是 在单独分离的心肌纤维中研究， 常规和电压钳记录。 生化 CAP对心脏作用的基础也将通过以下方式进行研究： 测量CAP诱导的细胞内 环核苷酸和/或肌醇磷酸。