A Digital DNA Nano Writer (DNA NanoFab)

数字 DNA 纳米写入器 (DNA NanoFab)

• 批准号: EP/L015668/1
• 负责人: Leroy Cronin
• 金额: $ 30.61万
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FL015668%2F1
• 关键词: Digital; DNA; Nano; Writer; NanoFab

Today gene sequencing is dropping in price with the $1000 dollar genome a real possibility but what about the direct writing of DNA? Progress in DNA sequencing is down to novel developments in electronic 'read' technology but currently there is no direct 'write'. Indirect methods such as DNA or peptide synthesis are improving but these are 'bulk' methods and sub-unit control on individual molecular strands is not achieved. In this bright idea we will build a hybrid, digital CMOS driven synthetic chemical platform to write DNA directly taking advantage of the speed and parallelism of CMOS aiming for GHz write rates on a single strand of DNA and scale up using PCR. Not only will this allow the 'direct' writing of DNA but also of proteins and polymer chains. This idea is transformative since the end result will be a system that allows the digital control of matter combining digital synthesis and chemical read-write steps. As such, this approach will realise the dream of a molecular assembler allowing access to synthetic DNA an order of magnitude longer in length than the current state of the art, as inexpensively and as quickly as sequence data. In developments beyond DNA, application to polymer science will allow the assembly of digitally defined polymer strands subunit by subunit allowing a nanopore assembler to be used for the world's first true digital matter fabricator or nano-assembler.

今天，基因测序的价格正在下降，1000美元的基因组成为可能，但DNA的直接书写呢？DNA测序的进步归功于电子“读取”技术的新发展，但目前还没有直接的“写入”技术。DNA或肽合成等间接方法正在改进，但这些是“批量”方法，并且无法实现对单个分子链的亚基控制。在这个聪明的想法中，我们将建立一个混合的，数字CMOS驱动的合成化学平台，直接写入DNA，利用CMOS的速度和并行性，在单链DNA上实现GHz的写入速率，并使用PCR进行扩展。这不仅可以“直接”编写DNA，还可以编写蛋白质和聚合物链。这个想法是革命性的，因为最终的结果将是一个系统，允许数字控制物质结合数字合成和化学读写步骤。因此，这种方法将实现分子组装器的梦想，使合成DNA的长度比目前的技术水平长一个数量级，与序列数据一样便宜和快速。在DNA之外的发展中，聚合物科学的应用将允许一个亚基一个亚基地组装数字定义的聚合物链，从而使纳米孔组装器用于世界上第一个真正的数字物质制造或纳米组装器。

项目成果

Investigating the Transformations of Polyoxoanions Using Mass Spectrometry and Molecular Dynamics.

使用质谱和分子动力学研究了多氧疗法的转化。

• DOI: 10.1021/jacs.6b02245
• 发表时间: 2016-07-20
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Cameron JM;Vilà-Nadal L;Winter RS;Iijima F;Murillo JC;Rodríguez-Fortea A;Oshio H;Poblet JM;Cronin L
• 通讯作者: Cronin L

An autonomous organic reaction search engine for chemical reactivity.

• DOI: 10.1038/ncomms15733
• 发表时间: 2017-06-09
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Dragone V;Sans V;Henson AB;Granda JM;Cronin L
• 通讯作者: Cronin L

Networking chemical robots for reaction multitasking.

• DOI: 10.1038/s41467-018-05828-8
• 发表时间: 2018-08-24
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Caramelli D;Salley D;Henson A;Camarasa GA;Sharabi S;Keenan G;Cronin L
• 通讯作者: Cronin L

Discovering New Chemistry with an Autonomous Robotic Platform Driven by a Reactivity-Seeking Neural Network.

• DOI: 10.1021/acscentsci.1c00435
• 发表时间: 2021-11-24
• 期刊: ACS central science
• 影响因子: 18.2
• 作者: Caramelli D;Granda JM;Mehr SHM;Cambié D;Henson AB;Cronin L
• 通讯作者: Cronin L

Taming the Combinatorial Explosion of the Formose Reaction via Recursion within Mineral Environments

• DOI: 10.1002/syst.201900014
• 发表时间: 2019-09-01
• 期刊: CHEMSYSTEMSCHEM
• 作者: Colon-Santos, Stephanie;Cooper, Geoffrey J. T.;Cronin, Leroy
• 通讯作者: Cronin, Leroy