An electronic-based ELISA combined with microfluidics

基于电子的 ELISA 与微流体技术相结合

• 批准号: EP/L020920/1
• 负责人: Themis Prodromakis
• 金额: $ 110.8万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FL020920%2F1
• 关键词: electronic; based; ELISA; combined; microfluidics

Our aim is to develop a low-cost real-time protein detection device to continuously monitor cytokines during in-vitro culture that could eventually expand in clinical practice. We will do this by exploiting discrete electronic components as chemical sensors that are compatible with unique microfluidic chips for minimising the overall cost of the device in combination with small size enzyme-linked immunosorbent assay (ELISA) chambers that minimise antibody requirements. We aspire achieving this by adapting well established manufacturing techniques, currently employed in fabricating printed-circuit boards (PCBs), that could effortlessly render bespoke functionalised electrodes coupled with um-scale fluidic channels/chambers.

我们的目标是开发一种低成本的实时蛋白质检测设备，在体外培养过程中连续监测细胞因子，最终可以扩展到临床实践。我们将通过利用分立的电子元件作为化学传感器来实现这一目标，这些元件与独特的微流体芯片兼容，以最大限度地降低设备的整体成本，并结合小尺寸的酶联免疫吸附测定（ELISA）室，以最大限度地减少抗体需求。我们希望通过调整目前用于制造印刷电路板（PCB）的成熟制造技术来实现这一目标，这些技术可以毫不费力地使定制的功能化电极与微米级流体通道/腔室耦合。

项目成果

Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs.

• DOI: 10.1038/s41598-018-30360-6
• 发表时间: 2018-08-10
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Sanzari I;Humphrey EJ;Dinelli F;Terracciano CM;Prodromakis T
• 通讯作者: Prodromakis T

A Novel Microfluidic Point-of-Care Biosensor System on Printed Circuit Board for Cytokine Detection.

• DOI: 10.3390/s18114011
• 发表时间: 2018-11-17
• 期刊: Sensors (Basel, Switzerland)
• 作者: Evans D;Papadimitriou KI;Vasilakis N;Pantelidis P;Kelleher P;Morgan H;Prodromakis T
• 通讯作者: Prodromakis T

Towards a smartphone-aided electronic ELISA for real-time electrochemical monitoring

用于实时电化学监测的智能手机辅助电子 ELISA

• DOI: 10.1109/iscas.2017.8050616
• 发表时间: 2017
• 作者: Pechlivanidis N

A PCB-based electronic ELISA system for rapid, portable infectious disease diagnosis

• DOI: 10.1109/biocas.2016.7833779
• 发表时间: 2016-10
• 期刊: 2016 IEEE Biomedical Circuits and Systems Conference (BioCAS)
• 作者: K. Papadimitriou;Daniel Evans;H. Morgan;T. Prodromakis

Tissue engineering techniques in cardiac repair and disease modelling.

心脏修复和疾病建模中的组织工程技术。

• DOI: 10.2174/13816128113199990442
• 发表时间: 2014
• 期刊: Current pharmaceutical design
• 影响因子: 3.1
• 作者: Rao C