DISPERSION OF DIFFUSIBLE INDICATORS IN THE BODY

计划的重点是小分子溶质的传输机制。 从血液进入心肌，特别是在 跨毛细管壁转运的表征，通过反式和 内皮细胞间的路径。以前的工作已经有很长一段时间了 获得血管内和血管内的清晰数学描述 跨毛细管运输。新的重点是在机制上 运输，尤指脂肪酸和腺苷进入或穿过的运输 内皮细胞通过促进或被动机制。多面性 将采用指示剂稀释法进行单向评价 腺苷和脂肪酸的质膜通量。具体实验 将会测试一种假设，即膜转运蛋白 腺苷和肌苷可以起到促进逆向转运蛋白的作用， IS，显示顺式到反式通量的增加，通过提高 跨浓度。该计划应产生经过验证的新技术 用于估计完整器官和血管内皮细胞壁上的通量 提供狗和兔心肌中特定溶质的数据。

The proposed program is focused on mechanisms of transport of small solutes from the blood into the myocardium and in particular on the characterization of transport across the capillary wall, via trans- and inter-endothelial cell routes. Previous work has gone a long way in obtaining clear mathematical descriptions of intravascular and transcapillary transport. The new emphasis is on the mechanisms of transport, particularly for fatty acids and adenosine into or across endothelial cells via facilitated or passive mechanisms. The multiple indicator dilution technique will be used to evaluate the unidirectional plasmalemmal fluxes of adenosine and fatty acids. Specific experiments will be done to test the hypothesis that the membrane transporters for adenosine and inosine can behave as facilitating countertransporters, that is, show augmentation of cis to trans flux by raising the trans-concentrations. The program should result in proven new techniques for estimating fluxes across endothelial cell walls in intact organs and provide data on specific solutes in the myocardium of dogs and rabbits.