DISPERSION OF DIFFUSIBLE INDICATORS IN THE BODY

体内弥散性指标的分散

• 批准号: 3485626
• 负责人: JAMES B BASSINGTHWAIGHTE
• 金额: $ 12.33万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3485626
• 关键词: capillary; diffusion; dogs; electron microscopy; fatty acid transport; fluidity; glucose transport; hemodynamics; indicator dilution test; ion transport; laboratory rabbit; mathematical model; passive transport; radiotracer; sarcolemma; solute; vascular endothelium permeability

The proposed program is focused on mechanisms of transport of small solutes from the blood into the myocardium and in particular on the characterization of transport across the capillary wall, via trans- and inter-endothelial cell routes. Previous work has gone a long way in obtaining clear mathematical descriptions of intravascular and transcapillary transport. The new emphasis is on the mechanisms of transport, particularly for fatty acids and adenosine into or across endothelial cells via facilitated or passive mechanisms. The multiple indicator dilution technique will be used to evaluate the unidirectional plasmalemmal fluxes of adenosine and fatty acids. Specific experiments will be done to test the hypothesis that the membrane transporters for adenosine and inosine can behave as facilitating countertransporters, that is, show augmentation of cis to trans flux by raising the trans-concentrations. The program should result in proven new techniques for estimating fluxes across endothelial cell walls in intact organs and provide data on specific solutes in the myocardium of dogs and rabbits.

拟议计划的重点是小溶质的运输机制 从血液进入心肌，特别是在 通过反式和 内皮细胞间途径。 之前的工作已经取得了很大的进展 获得血管内和血管内的清晰的数学描述 跨毛细血管运输。 新的重点是机制 运输，特别是脂肪酸和腺苷进入或跨越 内皮细胞通过促进或被动机制。 多重 指示剂稀释技术将用于评估单向 腺苷和脂肪酸的质膜通量。 具体实验 将进行以检验膜转运蛋白的假设 腺苷和肌苷可以充当促进反转运蛋白， 是，通过提高顺式到反式通量的增加 反式浓度。 该计划应该产生经过验证的新技术 用于估计完整器官中跨内皮细胞壁的通量 提供狗和兔子心肌中特定溶质的数据。