NADH FLUORESCENCE IMAGING IN HEART

心脏 NADH 荧光成像

• 批准号: 3508580
• 负责人: Clyde H Barlow
• 金额: $ 16.01万
• 依托单位: BARLOW SCIENTIFIC, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3508580
• 关键词: bioenergetics; chemical synthesis; clinical biomedical equipment; computer program /software; fluorescence; fluorimetry; image processing; laboratory rat; myocardial ischemia /hypoxia; nicotinamide adenine dinucleotide; oxygen tension; palladium; phosphorescence; porphyrins; video recording system

The long term goal of this research is to develop quantitative optical imaging methods for tissue bioenergetic studies. In Phase I, a videofluorometer with high spatial resolution was developed that produces NADH fluorescence images compensated for internal absorption due to blood volume changes. This device was used to evaluate both local and global ischemia in buffer and blood perfused rat hearts. Initial studies performed during Phase I demonstrate that an enhanced instrument can produce complimentary images of tissue oxygen tension using unique palladium porphyrins. Phase II will consist of: (1) upgrading the videofluorometer to obtain sequential NADH and palladium porphyrin emission images, (2) synthesizing appropriate palladium porphyrins and evaluating their utility for in vivo use, (3) developing software to produce tissue oxygen concentration images, and (4) calibrate and evaluate the use of this enhanced videofluorometer in studies of models of hypoxia and ischemia. This dual function videofluorometer in Phase II will allow rapid, sequential imaging of tissue metabolism and oxygen concentration and thus have much greater utility in spatially quantifying tissue metabolism and oxygen supply/demand for research purposes and potential clinical uses than current devices.

这项研究的长期目标是发展定量的 用于组织生物能量研究的光学成像方法。 同相 一是研制了一台高空间分辨率的视频荧光计 产生NADH荧光图像， 由于血容量的变化而吸收。 使用该器械 评价缓冲液和血液中的局部和整体缺血 灌流的大鼠心脏。 I期期间进行的初始研究 证明一个强化的工具可以产生互补性， 使用独特的钯卟啉的组织氧张力图像。 第二阶段将包括：（1）升级视频荧光计， 获得连续的NADH和钯卟啉发射图像， (2)合成合适的钯卟啉并评价 它们在体内使用的效用，（3）开发软件以产生 组织氧浓度图像，以及（4）校准和评估 使用这种增强的视频荧光计在模型的研究， 缺氧和缺血。 该双功能视频荧光计在第二阶段将允许快速， 组织代谢和氧浓度的顺序成像 因此在空间量化组织方面具有更大的效用 代谢和氧气供应/需求的研究目的， 潜在临床用途。