BIOLOGICAL MATERIALS SEPARATION BY CONDUCTIVE MEMBRANES

描述：(改编自申请人的摘要)膜技术 因为生物分离在过去几年中发展迅速。这个 新型聚合物材料和高度不对称膜的开发 这一演变的两个主要原因。尽管有这些进步， 然而，使用基于膜的过程进行生物分离仍然是 受蛋白质造成的膜污染和凝胶形成的限制 吸附。这些问题严重限制了膜的效率。 浓缩产品的过滤过程，实际上不允许 使用膜进行更复杂的分离，如尺寸选择 组分和生物特异性吸附。 近几年来国内外研制电导电膜的研究报告 一种控制渗透的电势垒的制备 带电物种拓宽了潜在的分离过程。在这 计划，先进表面技术(AST)将描述创新的新 提高生物净化效果的技术途径 材料。 申请者将比较导电的工作特性， 带电薄膜与标准非导电薄膜的对比 确定表观孔径、保留特性和 吞吐速率。导电膜效应的进一步研究 两种单抗的分离及细胞存活率 哺乳动物和非哺乳动物细胞，将被启动。

DESCRIPTION: (Adapted from the applicant's abstract) Membrane technology for bioseparation has evolved rapidly over the last few years. The development of new polymeric materials and highly asymmetric membranes are two of the major reasons for this evolution. Despite these advances, however, the use of membrane-based processes for bioseparations is still limited by both membrane fouling and gel formation due to protein adsorption. These problems severely limit the efficiency of membrane filtration processes for concentrating products, and virtually disallow the use of membranes for more sophisticated separations such as size selective fraction and biospecific sorption. Recent reports on the investigation of formulating conductive membranes for the preparation of an electric potential barrier to control permeation of the charged species have broadened potential separation processes. In this program, Advanced Surface Technology (AST) will describe innovative new technological approaches for enhancing the purification of biological materials. The applicants will compare the operating characteristics of conductive, electrically charged membranes against standard, non-conductive membranes to determine changes in apparent pore size, retention characteristics and throughput rates. Additional studies of the effects of conductive membrane separation on monoclonal antibodies as well as cell viability for both mammalian and non-mammalian cells, will be initiated.