BIOLOGICAL MATERIALS SEPARATION BY CONDUCTIVE MEMBRANES

通过导电膜分离生物材料

• 批准号: 3498516
• 负责人: IH-HOUNG LOH
• 金额: $ 5万
• 依托单位: AST PRODUCTS, INC.
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3498516
• 关键词: biomaterials; electrical conductance; membrane; membrane activity; membrane permeability; membrane reconstitution /synthesis; physical separation; polymers

DESCRIPTION: (Adapted from the applicant's abstract) Membrane technology for bioseparation has evolved rapidly over the last few years. The development of new polymeric materials and highly asymmetric membranes are two of the major reasons for this evolution. Despite these advances, however, the use of membrane-based processes for bioseparations is still limited by both membrane fouling and gel formation due to protein adsorption. These problems severely limit the efficiency of membrane filtration processes for concentrating products, and virtually disallow the use of membranes for more sophisticated separations such as size selective fraction and biospecific sorption. Recent reports on the investigation of formulating conductive membranes for the preparation of an electric potential barrier to control permeation of the charged species have broadened potential separation processes. In this program, Advanced Surface Technology (AST) will describe innovative new technological approaches for enhancing the purification of biological materials. The applicants will compare the operating characteristics of conductive, electrically charged membranes against standard, non-conductive membranes to determine changes in apparent pore size, retention characteristics and throughput rates. Additional studies of the effects of conductive membrane separation on monoclonal antibodies as well as cell viability for both mammalian and non-mammalian cells, will be initiated.

描述：（改编自申请人摘要）膜技术 在过去的几年里，生物分离技术发展迅速。 的 开发新的聚合物材料和高度不对称的膜， 这是这一演变的两大原因。 尽管取得了这些进展， 然而，使用基于膜的方法进行生物分离仍然是困难的。 受到膜污染和蛋白质引起的凝胶形成的限制 吸附作用 这些问题严重限制了膜的效率 浓缩产品的过滤过程，几乎不允许 使用膜进行更复杂的分离，例如尺寸选择性分离 分馏和生物特异性吸附。 最近的报告，研究制定导电膜， 制备电势势垒以控制 带电物质具有加宽的电势分离过程。 在这 计划，先进的表面技术（AST）将描述创新的新 提高生物制品净化能力的技术途径 材料. 本申请人将比较导电的， 带电膜与标准非导电膜的对比 以确定表观孔径、保留特性和 吞吐率。 导电膜效应的补充研究 单克隆抗体的分离以及细胞活力 哺乳动物和非哺乳动物细胞，将被启动。