Degradation of vascular devices (de-vasc): a combined engineering and biological approach

血管装置的降解（de-vasc）：工程和生物学相结合的方法

• 批准号: EP/P009662/1
• 负责人: Michael Bryant
• 金额: $ 12.87万
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FP009662%2F1
• 关键词: Degradation; vascular; devices; de; vasc

The PI has established a multi-disciplinary team of engineers and biologists with the view to develop novel methodologies to understand the metal-vascular biology interface that is established during stent or graft placement using core underpinning engineering and biological sciences. Expandable metallic structures (typically Co-based or NiTi alloys), known as 'stents', are commonly used to treat Peripheral Arterial Occlusive Diseases (PAOD), a thickening of the artery wall as a result of proliferation of intimal-smooth-muscle cells and Abdominal Aortic Aneurysms (AAA), a weakening of the aorta. PAOD and AAA are commonly treated with braided or a series of overlapping stents resulting in multiple metal-metal contacts being established in-vivo. A thorough understanding of the degradation processes and their role on the remodeling of the vasculature does not exist. This project will fuse contemporary tribocorrosion methods with unique patient specific cell cultures (ie cells obtained from a human donor undergoing vascular intervention) to quantify the synergy between mechanical, corrosion and biological approaches. Novel surface science approaches will then be explored, using the developed methodologies, with the view to further understand and increase the bio-compatibility of future implanted devices. The framework established as part of this grant will bridge the current gap in bio-compatibility testing.The development of an instrument combining mechanical and corrosive processes with clinically relevant biology (all of which are present and interacting in-vivo) will provide an alternative to simplistic static cell culture and complex animal models. This has the potential to significantly reduce R&D costs through more effective screening of prospective technologies as well as reducing the need for animal testing.

主要研究者建立了一个由工程师和生物学家组成的多学科团队，旨在开发新方法，以了解在支架或移植物放置过程中使用核心基础工程和生物科学建立的金属-血管生物学界面。可扩张金属结构（通常为钴基或镍钛合金），称为“支架”，通常用于治疗外周动脉闭塞性疾病（PAOD），一种由于内膜平滑肌细胞增殖导致的动脉壁增厚和腹主动脉瘤（AAA），一种主动脉弱化。PAOD和AAA通常使用编织支架或一系列重叠支架进行治疗，从而在体内建立多个金属-金属接触。对降解过程及其在血管重塑中的作用还没有彻底的了解。该项目将融合当代摩擦腐蚀方法与独特的患者特异性细胞培养物（即从接受血管干预的人类供体中获得的细胞），以量化机械，腐蚀和生物方法之间的协同作用。然后将使用开发的方法探索新的表面科学方法，以进一步了解和提高未来植入器械的生物相容性。作为该资助的一部分建立的框架将弥合目前生物相容性测试的差距，开发一种将机械和腐蚀过程与临床相关生物学（所有这些都存在并在体内相互作用）相结合的仪器，将为简单的静态细胞培养和复杂的动物模型提供替代方案。这有可能通过更有效地筛选有前景的技术以及减少对动物试验的需求来大幅降低研发成本。

项目成果

Tribological Characteristics of Human Vascular Smooth Muscle Cells: The Implication of Disease State on Friction

• DOI: 10.1016/j.biotri.2020.100122
• 发表时间: 2020-06
• 期刊: Biotribology
• 作者: Emily R. Clark;Karen E. Hemmings;S. Greco;A. Neville;K. Porter;M. Bryant

Fretting-corrosion of cardiovascular stent materials: The role of electrochemical polarisation on debris generation mechanisms

• DOI: 10.1016/j.biotri.2019.100093
• 发表时间: 2019-06
• 期刊: Biotribology
• 作者: Emily R. Clark;K. Porter;M. Bryant