ISOTOPE-ASSISTED DIFFERENTIAL METABOLOMICS

同位素辅助差异代谢组学

• 批准号: 8361153
• 负责人: JOHN LUTE MARKLEY
• 金额: $ 1.52万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361153
• 关键词: Arabidopsis; Biological; Cataloging; Catalogs; Chemicals; Coupled; Databases; Funding; Future; Grant; Isotopes; Mass Spectrum Analysis; Monitor; NMR Spectroscopy; National Center for Research Resources; One-Step dentin bonding system; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Source; Stable Isotope Labeling; Structure; Technology; Time; United States National Institutes of Health; cost; high throughput analysis; human tissue; metabolomics; novel strategies; small molecule

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Obtain additional spectral information from representatives of the 100 important metabolites of Arabidopsis and populate the database. In the future, we will have a catalog containing the structures of the 10,000 or more' most-abundant small molecules in all human tissues. We will also be able to use this information to monitor their fluctuations in concentration in real time, as our bodies continually change. The purpose of this proposal is to take us one step closer to creating metabolomic technologies to fill this void. This proposal explores the use of stable isotope labeling of biological samples coupled with the analytical high sensitivity of mass spectrometry (MS) and the chemical selectivity of high-field NMR spectroscopy as enabling technologies for high-throughput analysis of metabolomes. We call this novel approach isotope assisted metabolomics.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 从100个重要代谢物的代谢物的代表中获取其他光谱信息，并填充数据库。将来，我们将拥有一个目录，其中包含所有人类组织中10,000个或更多“最丰富的小分子的结构”。随着身体的不断变化，我们还将能够使用此信息实时监测其集中注意力的波动。该提案的目的是使我们更近一步，以创建代谢组技术来填补这一空白。该建议探讨了将生物样品的稳定同位素标记与质谱分析高灵敏度（MS）结合使用的稳定同位素标记，以及高场NMR光谱法的化学选择性作为促成技术，用于对代谢组的高直发分析。我们称这种新颖的方法同位素辅助代谢组学。