METABOLIC RESPONSES IN E COLI TO OSMOTIC STRESS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 当细胞在高渗透压的介质中培养时，会积累有机渗透物质。最近的证据表明，野生型大肠杆菌(如泌尿系致病菌株CFT073)具有渗透调节转运蛋白并积累渗透调节物质，这在对大肠杆菌K-12的研究中并不明显。正在使用核磁共振和质谱学进行这些观察，以努力识别以前未知的渗透调节物质，这些渗透调节物质在缺乏已知渗透调节系统的渗透压应激细菌中积累到高水平。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Cells accumulate organic osmolytes when cultured in high osmolality media. Recent evidence indicates that wild type E. coli (eg uropathgenic strain CFT073) possess osmoregulatory transporters and accumulate osmolytes that have not become evident from studies of E. coli K-12. These observations are being pursued using NMR and Mass Spectrometries in an effort to identify previously unknown osmolytes that accumulate to high levels in osmotically stressed bacteria that lack known osmoregulatory systems.