ISOTOPE-ASSISTED DIFFERENTIAL METABOLOMICS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 从拟南芥的100种重要代谢物的代表中获得额外的光谱信息并填充数据库。在未来，我们将拥有一个包含所有人体组织中10，000或更多“最丰富的小分子”结构的目录。我们还将能够利用这些信息来监测它们在真实的时间内的浓度波动，因为我们的身体在不断变化。这项提议的目的是让我们更接近创造代谢组学技术来填补这一空白。该提案探讨了使用稳定同位素标记的生物样品加上分析的高灵敏度的质谱（MS）和化学选择性的高场NMR光谱作为使能技术的代谢组的高通量分析。我们称这种新方法为同位素辅助代谢组学。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Obtain additional spectral information from representatives of the 100 important metabolites of Arabidopsis and populate the database. In the future, we will have a catalog containing the structures of the 10,000 or more' most-abundant smallmolecules in all human tissues. We will also be able to use this information to monitor their fluctuations in concentration in real time, as our bodies continually change. The purpose of this proposal is to take us one step closer to creating metabolomic technologies to fill this void. This proposal explores the use of stable isotope labeling of biological samples coupled with the analytical high sensitivity of mass spectrometry (MS) and the chemical selectivity of high-field NMR spectroscopy as enabling technologies for high-throughput analysis of metabolomes. We call this novel approach isotopeassisted metabolomics.