ISOTOPE ASSISTED METABOLOMICS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 同位素辅助差异代谢组学：全球代谢谱高通量分析的新策略。在过去的10年里，人们致力于开发高通量的方法来分析生物样品中的代谢物。虽然我们可以用质谱仪(MS)检测浓度低至10-18摩尔的分子，并可以用核磁共振(NMR)区分密切相关的物种，但样品的复杂性严重限制了这些技术在代谢组学研究中的表现。我们开发了一种新的基于重同位素的策略，将与传统方法相关的问题降至最低，并扩大了全球代谢图谱的范围和重复性。我们目前正在使用这种方法来研究拟南芥基因敲除的抗旱机制，并阐明人类红细胞代谢通量的调节。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Isotope-Assisted Differential Metabolomics: a New Strategy for High Throughput Analysis of Global Metabolic Profiles. Over the past 10 years, a considerable effort has been devoted to developing high throughput methods for profiling metabolites in biological samples. Although we can detect molecules at concentrations as low as 10-18 molar with mass spectrometry (MS) and can differentiate between closely related species using nuclear magnetic resonance (NMR), sample complexity has severely limited the performance of these techniques in metabolomics studies. We have developed a new heavy-isotope based strategy that minimizes problems associated with traditional methods and extends the scope and reproducibility of global metabolic profiling. We are currently using this approach to investigate the mechanism of drought resistant in genetic knockouts of Arabidopsis thaliana and to elucidate the regulation of metabolic flux in human erythrocytes.