MINNESOTA FETAL TISSUE BANK

已经提出了人胎儿组织的移植， 治疗各种毁灭性的疾病和紊乱。 的 细胞移植疗法的潜在临床应用， 然而，由于供应有限， 这样的组织用于研究。 我们建议评估质量和数量 仅从自然流产中获得的人类胎儿组织， 异位妊娠作为移植治疗的来源。 我们 进一步建议制定和测试方法， 建立有效的人类胎儿组织库。 这些建议 将通过以下项目和具体目标加以解决。 项目1将制定和评估采购、加工、 并分配人类胎儿组织， 最高质量的细胞移植。 项目2将评估 一项前瞻性的人类胎儿组织用于组织库的可用性 一项涉及明尼阿波利斯/圣保罗大都会区当地医院的研究 在涉及当地医院和卫生部门的回顾性研究中， 维护组织（HMO）。 项目3将评估新的方法， 储存和保存人类胎儿组织用于组织库。 项目4 将表征人类造血祖细胞，用于 移植 项目5将评估胎儿细胞的发育 体液免疫系统。 项目6将确定胎儿标志物 胰岛祖细胞，并确定其生长潜力， 这些细胞受到生长因子的影响。 最后，项目7 将研究表皮生长因子对增殖的影响， 胎儿多巴胺神经细胞，并评估移植的能力 中脑神经元释放多巴胺，支配宿主大脑， 恢复运动功能 这些项目将共同评估 从自然流产中获得的各种细胞的活力，或 异位妊娠，并确定这种组织的可行性， 作为移植治疗的来源。

The transplantation of human fetal tissue has been proposed for the treatment of a variety of devastating diseases and disorders. The potential clinical applications of cell transplantation therapies, however, have been significantly hampered by the limited availability of such tissue for research. We proposed to assess the quality and quantity of human fetal tissues obtained solely from spontaneous abortions and ectopic pregnancies to serve as sources for transplantation therapy. We further propose to develop and test methods that will be essential for the establishment of effective human fetal tissue banks. These proposals will be addressed by the following projects and specific aims. Project 1 will develop and evaluate methods for procuring, processing, and distributing human fetal tissue which will ensure tissue of the highest quality for cellular transplantation. Project 2 will assess the availability of human fetal tissue for tissue banking in a prospective study involving local hospitals in the Minneapolis/St. Paul metropolitan area, and in retrospective studies involving local hospitals and health maintenance organizations (HMOs). Project 3 will evaluate new ways of storing and preserving human fetal tissue for tissue banking. Project 4 will characterize human hematopoietic progenitors cells for use in transplantation. Project 5 will evaluate the development of fetal cells of the humoral immune system. Project 6 will determine markers for fetal pancreatic islet progenitor cells, and determine the growth potential of these cells under the influence of growth factors. Finally, Project 7 will examine the effects of epidermal growth factor on the proliferation of fetal dopamine nerve cells, and assess the ability of transplanted mesencephalic neurons to release dopamine, innervate the host brain, and restore locomotor function. Together these projects will assess the viability of a variety of cells obtained from spontaneous abortions or ectopic pregnancies, and determine the feasibility of such tissue to serve as sources for transplantation therapies.