ENDOCRINE EFFECTS OF ALCOHOL ABUSE

这项研究计划的主要目标是阐明 导致生育率下降的机制 由酗酒的男性经历的。主要的假设是 测试的是酒精和/或乙醛(乙醇是第一个 代谢物)或乙醇或 乙醛直接作用于支持细胞 导致支持细胞失败的功能障碍 供应充足的铁，雄激素结合蛋白，铜蓝蛋白， 铜和其他材料给体内发育中的生殖细胞 生精小管。 本研究将使用的工具包括：放射免疫分析， 酶分析，受体结合研究，核磁共振波谱和 成像与特定信使的分离和定量 核糖核酸物种。大部分研究将在 分离大鼠支持细胞。少数研究将是 在体内使用酒精喂养的大鼠和使用一种 等热量喂养对照组大鼠。 适用于预防和/或治疗的新信息 与酒精滥用相关的不孕症将发展为 这项研究的结果。

This research program has as its primary goal the elucidation of the mechanisms responsible for the reduction in fertility experienced by alcohol abusing males. The major hypothesis to be tested is that' alcohol and/or acetaldehyde (ethanol's first metabolic product) or the metabolism of either ethanol or acetaldehyde are immediately responsible for a Sertoli cell dysfunction that leads to failure of the Sertoli cells to adequately supply iron, androgen binding protein, ceruloplasmin, copper and other materials to the developing germ cells within the seminiferous tubules. The tools to be used in this research include: radioimmunoassay, enzyme assay, receptor binding studies, NMR spectroscopy and imaging and the isolation and quantitation of specific messenger RNA species. The majority of the studies will be performed in isolated rat Sertoli cells. A minority of studies will be performed in vivo using the alcohol fed rat and the use of an isocalorically fed control rat. New information applicable to the prevention and/or treatment of alcohol abuse associated infertility will be developed as a result of this research.