ENDOCRINE EFFECTS OF ALCOHOL ABUSE

这项研究计划的主要目标是阐明 导致生育率下降的机制 酒精滥用男性的经验。 主要的假设是 酒精和/或乙醛（乙醇的第一个 代谢产物）或乙醇或 乙醛直接导致支持细胞 导致支持细胞功能障碍， 充分供应铁、雄激素结合蛋白、铜蓝蛋白， 铜和其他物质对发育中的生殖细胞 生精小管 在这项研究中使用的工具包括：放射免疫测定， 酶测定、受体结合研究、NMR光谱和 成像和特定信使的分离和定量 RNA种类 大多数研究将在 分离的大鼠睾丸支持细胞。 少数研究将 使用酒精喂养的大鼠在体内进行， 等热量喂养对照大鼠。 适用于预防和/或治疗 酒精滥用相关的不孕症将发展为 这项研究的结果。

This research program has as its primary goal the elucidation of the mechanisms responsible for the reduction in fertility experienced by alcohol abusing males. The major hypothesis to be tested is that' alcohol and/or acetaldehyde (ethanol's first metabolic product) or the metabolism of either ethanol or acetaldehyde are immediately responsible for a Sertoli cell dysfunction that leads to failure of the Sertoli cells to adequately supply iron, androgen binding protein, ceruloplasmin, copper and other materials to the developing germ cells within the seminiferous tubules. The tools to be used in this research include: radioimmunoassay, enzyme assay, receptor binding studies, NMR spectroscopy and imaging and the isolation and quantitation of specific messenger RNA species. The majority of the studies will be performed in isolated rat Sertoli cells. A minority of studies will be performed in vivo using the alcohol fed rat and the use of an isocalorically fed control rat. New information applicable to the prevention and/or treatment of alcohol abuse associated infertility will be developed as a result of this research.