MOLECULAR GENETIC ANALYSIS OF LYMPHOCYTE FUNCTION

淋巴细胞功能的分子遗传学分析

• 批准号: 3768785
• 负责人: D H MARGULIES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3768785
• 关键词: MHC class I antigen; T cell receptor; antigen presentation; human immunodeficiency virus 1; lymphocyte; molecular genetics; natural killer cells; protein engineering; proteolysis; tissue /cell culture; virus protein

The focus of these studies has been on the functional role of the MHC class I molecule and the development of model systems for examining this function in vitro. Specific accomplishments during the past year include: 1) the characterization of proteolytic pathways involved in the extracellular processing of different class I restricted peptides; 2) the characterization of the minimal peptides derived from the HIV-1 gp 160 envelope glycoprotein that are presented by both H-2Dd and I-Ad; 3) the biochemical and functional analysis of a set of single chain class I molecules generated by engineering techniques; 4) the evaluation of the role of the MHC class I molecule H-2Dd in the signalling of a subset of NK cells bearing the cell surface marker Ly-49; 5) the evaluation of T cell receptor/class I interactions.

这些研究的重点一直集中在MHC的功能作用上 I类分子及其研究模型系统的发展 在体外发挥作用。过去一年的具体成就包括： 1)蛋白水解酶途径的特征 不同I类限制性多肽的胞外加工；2) HIV-1GP 160最小多肽的特性研究 H-2DD和I-Ad都呈现的包膜糖蛋白；3) 一组单链I类化合物的生化和功能分析 由工程技术产生的分子；4)评价 MHC-I类分子H-2DD在NK细胞亚群信号传导中的作用 带有细胞表面标记Ly-49的细胞；5)T细胞的评价 受体/I类相互作用。