IN VITRO MEASUREMENT OF BINDING OF SELF AND ANTIGENIC PEPTIDES TO MHC MOLECULES

自身肽和抗原肽与 MHC 分子结合的体外测量

• 批准号: 3790855
• 负责人: D H MARGULIES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790855
• 关键词: MHC class I antigen; X ray crystallography; antigens; autoantigens; chemical binding; intermolecular interaction; mathematical model; peptides

The focus of this laboratory has been the MHC class I molecule, classically considered a molecular heterodimer consisting of a 46 kd polymorphic heavy chain, exemplified by the murine H-2K, D, and L molecules (analogous to the human HLA-A, -B, and -C molecules), that is non-covalently assembled with the 12 kd monomorphic light chain, beta2-microglobulin. Our objectives in this project have been to examine the interaction of self and antigenic peptides to the MHC class I molecule using several different systems and to evaluate these interactions in three dimensional computer modeling studies based on crystallographic MHC structures.

这个实验室的重点是MHC I类分子， 传统上认为是由46 kd的 多态性重链，例如鼠H-2K、D和L 分子（类似于人类HLA-A、-B和-C分子），即 与12 kd单态轻链非共价组装， β 2-微球蛋白 我们在这个项目中的目标是检查 自身和抗原肽与MHC I类分子的相互作用 使用几种不同的系统，并评估这些相互作用， 基于晶体学MHC的三维计算机模拟研究 结构.