IN VITRO MEASUREMENT OF BINDING OF SELF AND ANTIGENIC PEPTIDES TO MHC MOLECULES

自身肽和抗原肽与 MHC 分子结合的体外测量

• 批准号: 2566827
• 负责人: D H MARGULIES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2566827
• 关键词: MHC class I antigen; antigen antibody reaction; antigen presentation; autoantigens; chemical binding; immune response genes; immunoregulation; intermolecular interaction

The objective of this work has been to understand the details of the interaction of self and foreign antigenic peptides with the MHC class I molecule by detailed kinetic and equilibrium binding methods. In addition to allowing us to understand the underlying biochemical rules that govern protein/peptide interactions, these studies provide specific information on particular MHC/peptide interactions which govern the initiation of the immune response. In the past year we have further improved upon quantitative assays, examined a number of protein/peptide interactions, including antibody/peptide interactions. Understanding these processes on a biochemical and biophysical level provides a basis not only for understanding the binding reactions, but also offers an opportunity for rational design of peptide analogs that might be useful in immunization and intervention in autoimmune disease.

这项工作的目的是了解的细节， 自身和外源抗原肽与MHC I类的相互作用 分子通过详细的动力学和平衡结合方法。 在 除了让我们了解潜在的生物化学规则 控制蛋白质/肽相互作用，这些研究提供了具体的 关于特定MHC/肽相互作用的信息， 启动免疫反应。 在过去的一年里，我们进一步改进了定量分析， 研究了许多蛋白质/肽相互作用，包括 抗体/肽相互作用。 在A上理解这些过程 生物化学和生物物理水平不仅为 了解结合反应，但也提供了一个机会， 可能用于免疫的肽类似物的合理设计 和自身免疫性疾病的干预。