MOLECULAR GENETIC ANALYSIS OF LYMPHOCYTE FUNCTION

这个实验室的重点一直是MHC I类分子， 经典地认为是由46kd组成的分子杂二聚体 多态重链，以小鼠H-2K、D和L为例 分子(类似于人类的HLA-A、-B和-C分子)，即 与12kd的单晶轻链非共价组装， β2-微球蛋白。我们在这个项目标题下的目标是 评价：1)β2-微球蛋白和血清蛋白水解酶的影响 MHC-I类分子与MHC的功能和生化结合 抗原肽；2)结构域和功能域的表达和功能 重组DNA产生的I类分子亚区片段 方法，并在组织培养细胞中表达；3)功能 转基因小鼠模型系统中的可溶性MHC I类分子； 单链MHC I类分子的生产和功能。

The focus of this laboratory has been the MHC class I molecule, classically considered a molecular heterodimer consisting of a 46 kd polymorphic heavy chain, exemplified by the murine H-2K, D, and L molecules (analogous to the human HLA-A, -B, and -C molecules), that is non-covalently assembled with the 12 kd monomorphic light chain, beta2-microglobulin. Our objectives under this project title have been to evaluate: 1) the influence of beta2-microglobulin and serum proteases on functional and biochemical binding of MHC class I molecules to antigenic peptides; 2) the expression and function of domain and sub-domain fragments of class I molecules generated by recombinant DNA methods, and expressed in tissue culture cells; 3) the function of soluble MHC class I molecules in a transgenic mouse model system; and 4) the production and function of single chain MHC class I molecules.