MOLECULAR GENETIC ANALYSIS OF LYMPHOCYTE FUNCTION

淋巴细胞功能的分子遗传学分析

• 批准号: 3790745
• 负责人: D H MARGULIES
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790745
• 关键词: MHC class I antigen; T cell receptor; T lymphocyte; antigens; chemical binding; endopeptidases; gene expression; genetic manipulation; genetic translation; genetically modified animals; histocompatibility antigens; histocompatibility gene; hybridomas; immunogenetics; laboratory mouse; leukocyte activation /transformation; lymphocyte; major histocompatibility complex; membrane activity; membrane proteins; molecular cloning; molecular genetics; protein engineering; protein sequence; protein structure function; receptor binding; recombinant DNA; tissue /cell culture

The focus of this laboratory has been the MHC class I molecule, classically considered a molecular heterodimer consisting of a 46 kd polymorphic heavy chain, exemplified by the murine H-2K, D, and L molecules (analogous to the human HLA-A, -B, and -C molecules), that is non-covalently assembled with the 12 kd monomorphic light chain, beta2-microglobulin. Our objectives under this project title have been to evaluate: 1) the influence of beta2-microglobulin and serum proteases on functional and biochemical binding of MHC class I molecules to antigenic peptides; 2) the expression and function of domain and sub-domain fragments of class I molecules generated by recombinant DNA methods, and expressed in tissue culture cells; 3) the function of soluble MHC class I molecules in a transgenic mouse model system; and 4) the production and function of single chain MHC class I molecules.

这个实验室的重点是MHC I类分子， 传统上认为是由46 kd的 多态性重链，例如鼠H-2K、D和L 分子（类似于人类HLA-A、-B和-C分子），即 与12 kd单态轻链非共价组装， β 2-微球蛋白 我们在这个项目标题下的目标是 评价：1）β 2-微球蛋白和血清蛋白酶的影响 对MHC I类分子的功能和生物化学结合， 抗原肽; 2）结构域的表达和功能， 由重组DNA产生的I类分子的亚结构域片段 方法，并在组织培养细胞中表达; 3）功能 转基因小鼠模型系统中的可溶性MHC I类分子;和4） 单链MHC I类分子的产生和功能。