IN VITRO MEASUREMENT OF BINDING OF SELF AND ANTIGENIC PEPTIDES TO MHC MOLECULES

这项工作的目的是了解的细节， 自身和外源抗原肽与MHC I类的相互作用 分子通过详细的动力学和平衡结合方法。 这些 研究使我们能够理解潜在的生物化学规则， 控制肽/蛋白质相互作用，以及MHC分子如何结合 肽在细胞内区室以及细胞表面。 在过去的一年里，我们进一步开发了定量分析， 检查了许多肽/MHC以及几种肽抗体 交互. 了解这些过程的生化和 生物物理水平不仅为理解MHC 分子结合和选择自身和抗原肽，但也提供 合理设计肽类似物的机会， 免疫和用于自身免疫疾病干预。

The objective of this work has been to understand the details of the interaction of self and foreign antigenic peptides with the MHC class I molecule by detailed kinetic and equilibrium binding methods. These studies permit us to understand the underlying biochemical rules that govern peptide/protein interactions, as well as how MHC molecules bind peptides both in intracellular compartments as well as the cell surface. In the past year we have further developed quantitative assays and have examined a number of peptide/MHC as well as several peptide antibody interactions. Understanding these processes on a biochemical and biophysical level provides a basis not only for understanding how MHC molecules bind and select self and antigenic peptides, but also offers an opportunity for rational design of peptide analogs for both immunization and for intervention in autoimmune disease.