MOLECULAR GENETIC ANALYSIS OF LYMPHOCYTE FUNCTION

该实验室目前的重点是详细了解 细胞表面分子参与的结构/功能关系 免疫反应。特别是，我们在以下方面取得了重大进展： 过去一年在五个领域：a.使用分子工程技术 产生能够编码可溶性小鼠I类主要基因的基因 组织相容性复合体(MHC)抗原；B.使用纯化的可溶性 对来源于这些细胞的抗原进行生化和功能分析 可溶性H-2DD的行为；C.分析蛋白质的来源和功能 来源于I类抗原H-2DD和H-2DD的选择性剪接的mRNA H-2kb；D.分析I类化合物的结构-功能关系 分子通过详细的血清学、生化和功能研究 H-2DD/H-2LD嵌合蛋白；E.体外研究类的功能 II/I类嵌合基因。

The ongoing focus of the laboratory is to understand in detail the structure/function relationships of cell surface molecules involved in the immune response. In particular we have made significant progress over the past year in five areas: A. Using molecular engineering techniques to generate genes capable of encoding soluble murine class I major histocompatibility complex (MHC) antigens; B. Using the purified soluble antigens derived from these cells to analyze the biochemical and functional behavior of soluble H-2Dd; C. Analyzing the origin and function of proteins derived from alternatively spliced mRNAs of the class I antigens H-2Dd and H-2Kb; D. Analyzing the structure-function relationship of class I molecules by a detailed serological, biochemical and functional study of H-2Dd/H-2Ld chimeric proteins; E. Studying the function of in vitro class II/class I chimeric genes.