GENERATION OF MONOCLONALS TO HTLV II

HTLV-I和HTLV-II具有相似的基因组结构和相似的蛋白质 有65%的核酸同源性和70%的氨基酸同源性。我们 产生的小鼠抗HTLV-II病毒蛋白的单抗 含有核心蛋白(Gag)和包膜蛋白(Env)。免疫BALB/c小鼠 用提纯的HTLV-II病毒。采用酶联免疫吸附试验和免疫印迹法进行筛选 用于并确认阳性克隆。与核心发生反应的单克隆 鉴定了p24蛋白。这些单克隆体显示出优先 与HTLV-II p24发生反应，但与HTLV-I p24蛋白发生交叉反应。我们 还分离出了一种与M5H6发生特异性反应的单抗M5H6 HTLV-II的gp46囊膜蛋白。利用这一点进行蛋白质印迹分析 单抗对表观MR的弥漫性糖蛋白具有反应性 46,000只在HTLV-II感染细胞或纯化病毒中检测到 准备工作。M5H6单抗与重组蛋白反应 来源于HTLV-II gp46(AA 162-205)，将相关表位映射到 区域。免疫荧光检测M5H6与HTLV-II感染细胞结合。 还将介绍有关这些单气旋的其他研究。

HTLV-I and HTLV-II have similar genome organization and similar protein profiles sharing 65% nucleic acid homology and 70% amino acid homology. We generated murine monoclonal antibodies to HTLV-II viral proteins that react with core (gag) and envelope (env) proteins. BALB/c mice were immunized with purified HTLV-II virus. ELISA and Western blots were used to screen for and to confirm positive clones. Monoclonals that react with the core proteins p24 were identified. These monoclonals show preferential reactivity to HTLV-II p24 but cross-react with the HTLV-I p24 protein. We also isolated a monoclonal antibody, M5H6, which reacts specifically with the gp46 envelope protein of HTLV-II. Western blot analysis using this monoclonal showed reactivity to a diffuse glycoprotein of apparent Mr. of 46,000 which was detected only in HTLV-II infected cell or purified viral preparations. The M5H6 monoclonal reacted with a recombinant protein derived from HTLV-II gp46 (aa 162-205) mapping the relevant epitope to this region. M5H6 binds to HTLV-II infected cells in immunofluorescent assays. Other studies characterizing these monoclonals will be presented.