GENERATION OF MONOCLONALS TO HTLV II

我们正在生产针对HTLV II病毒蛋白的单抗，用于 用于HTLV II感染细胞的Western印迹和RIPA分析。老鼠 用被破坏的HTLV II洗涤剂免疫。 免疫后，小鼠放血，多克隆血清检测HTLV I和 HTLV II免疫印迹检测反应性。小鼠被处死，然后 脾细胞与融合伙伴融合产生杂交瘤细胞 台词。克隆了26个细胞系，并选择了用于研究的 细胞培养上清液对HTLV I和HTLV II的酶联免疫吸附试验。 初步结果表明，8个克隆的上清液与A 免疫印迹显示为HTLV II包膜糖蛋白的条带 污点。将对上清液进行进一步表征，以确定其 反应性。

We are generating monoclonal antibodies to HTLV II viral proteins for use in Western blot and RIPA analysis of HTLV II infected cells. Mice were immunized with detergent disrupted HTLV II. Following repeated boosts, the mice were bled and the polyclonal sera tested on HTLV I and HTLV II Western Blot for reactivity. The mice were sacrificed and spleen cells fused with a fusion partner to generate hybridoma cell lines. 26 cell lines were cloned and chosen for study based on the reactivity of the cell supernatant on HTLV I and HTLV II ELISA assays. Preliminary results show reactivity of supernatant from 8 clones with a band that appears to be the HTLV II envelope glycoprotein on Western blot. The supernatants will be further characterized to determine their reactivities.