CHARACTERIZATION OF A NOVEL HTLV-II CELL LINE

我们先前从一位再生障碍性贫血患者身上分离出一株细胞系(JR)。 它产生了HTLVII。我们已经确定了这个细胞系和病毒的特征 它产生了。此外，来自脐带血细胞的第二个细胞系 感染了JR病毒。该细胞系仍依赖于IL-2，并且 生长缓慢。Western印迹分析显示两种细胞系均为蛋白质 与其他HTLV-II病毒株相似的特征。Northern印迹分析 使用IL-2受体的细胞系表现出低水平的IL-2受体 RNA与其他感染细胞系的比较。我们正在调查使用 JR-脐带血细胞系作为HTLV早期感染模型的研究 我们对JR细胞系分离的研究正在为 出版。

We previously isolated a cell line (JR) from a patient with aplastic anemia that produces HTLVII. We have characterized this cell line and the virus it produces. In addition, a second cell line derived from cord blood cells infected with the JR virus. This cell line remains IL-2 dependent and grows slowly. Western blot analysis of the two cell lines shows protein profiles similar to other HTLV-II viral strains. Northern blot analysis of the cell lines used the IL-2 receptor, showed low levels of IL-2 receptor RNA compared to other infected cell lines. We are investigating the use of the JR-cord blood cell line as a model for early HTLV infection. Our studies on the isolation of JR cell line are being prepared for publication.