MOLECULAR GENETIC ANALYSIS OF RAS GENES

Ras基因被发现在高比例的人类肿瘤中被激活。 这些肿瘤的p21蛋白在关键的氨基酸改变 岗位 为了表征结构和功能域 我们已经使用了各种技术来突变这些ras基因， 基因. 使用寡核苷酸和接头的定点诱变 采用插入技术。 我们发现某些突变体 ras基因的转化能力。 当分析突变蛋白质时， 由于它们的生化特性，某些突变体不再能够 在体外测定中，自磷酸化。 我们正在分析这些变种人 表征p21蛋白的改变的生物化学性质。

Ras genes are found to be activated in a high proportion of human tumors. The p21 proteins from these tumors have amino acid alterations at critical positions. In order to characterize the structural and functional domains of the ras genes, we have used a variety of techniques to mutate these genes. Both site-directed mutagenesis using oligonucleotides and linker insertion techniques were employed. We found that certain mutants alter the transforming ability of ras genes. When mutated proteins were analyzed for their biochemical properties, certain mutants were no longer able to autophosphorylate in an in vitro assay. We are analyzing these mutants to characterize the altered biochemical properties of p21 proteins.