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HTLV-II ELISA AND WESTERN BLOTS

HTLV-II ELISA AND WESTERN BLOTS
HTLV-II ELISA 和蛋白质印迹
批准号：
3792602
负责人：
M RUTA
金额：
$0.0万
依托单位：
CENTER BIOLOGICS EVALUATION RESEARCH TRANSFUSION
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3792602
关键词：
antibody specificity antiviral antibody enzyme linked immunosorbent assay human T cell lymphotropic virus type 1 human T cell lymphotropic virus type 2 human tissue polymerase chain reaction western blottings

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中文摘要
翻译
我们已经建立了基于全病毒的HTLV II抗体的酶联免疫吸附试验 裂解液。提纯的HTLV II病毒被洗涤剂破坏并被用来包被 96个井牌。确定了最佳的蛋白质包衣水平。我们是 目前HTLV I/II阳性血清检测条件的优化 样本。结果表明，HTLV II的酶联免疫吸附试验结果良好。 对确认为HTLV I或HTLV I或HTLV I的样本的特异性和敏感性 2.聚合酶链式反应阳性。 我们还用Disrupt洗涤剂生成了HTLV II蛋白印迹 HTLV II病毒抗原。我们已经分析了它的区分能力 HTLV I和HTLV II印迹法检测感染者抗体 使用HTLV。
英文摘要
We have generated an ELISA for HTLV II antibody based on whole viral lysate. Purified HTLV II virus was detergent disrupted and used to coat 96 well plates. Optimum protein coating levels were determined. We are currently optimizing the conditions for testing HTLV I/II positive serum samples. Our results indicate that the HTLV II ELISA showed good specificity and sensitivity on samples that were confirmed as HTLV I or II positive by PCR. We have also generated an HTLV II Western blot using detergent disrupted HTLV II viral antigen. We have analyzed the differential ability of HTLV I and HTLV II blots to detect antibodies from patients infected with HTLV.
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会议论文
CHARACTERIZATION OF A NOVEL HTLV-II CELL LINE
FGF RECEPTOR EXPRESSION IN TUMORS
SEROLOGIC MARKERS OF HTLV-I/II INFECTION
MOLECULAR GENETIC ANALYSIS OF RAS GENES
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