CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

果蝇 ETS 和 ETS 样基因的特征

• 批准号: 3774851
• 负责人: D K WATSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3774851
• 关键词: Drosophilidae; antibody; binding proteins; developmental genetics; gene expression; genetic mapping; oncoproteins; protein sequence; protein structure function; protooncogene; transcription factor

D-elg (Drosophila ets-like gene) is a member of the Drosophila ets-related gene family that has extensive homology to the ets proto-oncogene. Drosophila represents an excellent system for the study of gene function because of the wealth of developmental, genetic, and molecular approaches available. D-elg serves as a promising gene to investigate ets-related gene function since it maps in a genetically well-characterized region of the Drosophila genome; this has facilitated the identification and analysis of D-elg mutants. Toward a goal of understanding the function of this ets-related gene in normal development, the molecular analysis of D- elg, a member of the Drosophila ets gene family, has been continued. The entire carboxy end of the D-elg protein has been overexpressed and used to generate antibodies to D-elg. D-elg shows significant homology to other ets proteins in the amino-terminal A domain and strong homology in the carboxy-terminal ETS domain. The D-elg protein is most similar to the alpha subunit of the mouse GA binding protein, and thus may represent the Drosophila homolog of this mouse transcription factor.

D-elg（Drosophila ets-like gene）是果蝇ets相关基因家族的一员 与ets原癌基因具有广泛同源性的基因家族。 果蝇是研究基因功能的一个极好的系统 由于发育、遗传和分子方法的丰富， available. d-elg是研究ets相关性的一个有希望的基因 基因功能，因为它映射在一个遗传特征良好的区域， 果蝇基因组;这有助于识别和 D-elg突变体的分析。 为了达到理解 这种与ets相关的基因在正常发育中，D- elg基因是果蝇ets基因家族的一员。 的 D-elg蛋白的整个羧基末端已经过表达，并用于 产生针对D-elg的抗体。 D-elg与其它的 ets蛋白在氨基末端A结构域和在氨基末端A结构域中的强同源性。 羧基末端ETS结构域。 D-elg蛋白与 小鼠GA结合蛋白的α亚基，因此可以代表小鼠GA结合蛋白的α亚基。 这种小鼠转录因子的果蝇同源物。