MODULATION OF ETS FUNCTION BY PROTEIN:PROTEIN INTERACTIONS

通过蛋白质：蛋白质相互作用调节 ETS 功能

• 批准号: 3853559
• 负责人: D K WATSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3853559
• 关键词: DNA binding protein; chimeric proteins; gene expression; genetic manipulation; genetic regulation; human genetic material tag; oncogenes; oncoproteins; open reading frames; protein purification

The human genome contains more than six ets-related genes )ETS1, ETS2, ERG, ERGB, ELK1, ELK2). In addition to having unique chromosomal locations, members of this ever-expanding gene family have unique patterns of expression. o understand the function(s) and mechanisms responsible for the functional differences between these genes, we intend to identify genes whose products are able to interact with ETS proteins. To achieve this goal, constructs containing defined segments of the open reading frames (ORF) from the human ETSI and ETS2 genes have been placed into prokaryotic vectors that allow for overexpression and rapid purification of the fusion protein produced by each construct. These vectors contain defined protein cleavage sites that allow for isolation of the unfused protein. These proteins will be labelled and utilized as probes to identify gene products (and the appropriate genes) with which they are able to interact. This methodology will be applied to other genes and will allow us to begin to dissect the role(s) of the ETS genes in the complex network of gene regulation and cellular signal transduc- tion.

人类基因组包含超过六个与ets相关的基因（ETS1，ETS2， ERG、ERGB、ELK 1、ELK 2）。 除了具有独特的染色体 这个不断扩大的基因家族的成员具有独特的 表达模式。o了解功能和机制 负责这些基因之间的功能差异，我们打算 以鉴定其产物能够与ETS蛋白相互作用的基因。 为了实现这一目标，构造包含开放的 来自人ETSI和ETS2基因的阅读框（ORF）已经被放置在 到原核载体，允许过表达和快速 纯化由各构建体产生的融合蛋白。 这些 载体含有限定的蛋白质切割位点， 未融合的蛋白质。 这些蛋白质将被标记并用作 探针，以确定基因产物（和适当的基因）， 它们能够相互作用。 这种方法将适用于其他 这将使我们开始剖析ETS基因的作用 在基因调控和细胞信号转导的复杂网络中， 是的。