CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

从人类到果蝇的生物体都被发现含有 与病毒同源的细胞序列和转录本- ONC基因。它们所编码的基因的正常功能 细胞序列是未知的。只有通过理解常态 这些细胞基因的功能(c-onc)我们是否有可能 意味着理解它们如何转变为 病毒的转导。考虑到这一目标，这项研究 开始使用果蝇。 与鸡Ets区同源的细胞序列 本实验室在果蝇体内发现了逆转录病毒E-26。 该基因的特征部分对应于后两个 与鸡c-ETS-1基因有90%以上的同源性。 预测的氨基酸水平。它被命名为D-ETS-2，并 定位在染色体3R的58A/B位置，并产生一种 所有发育阶段均为4.7kb的单一转录本。低 果蝇基因组DNA的严格杂交显示了几个 其他也与病毒ETS探针E1.28杂交的条带。 在这些条件下杂交一个cdna文库导致了 一个与v-1基因同源性较高的cDNA克隆的分离 ETS但不是D-ETS-2。这种基因被称为D-ELG，意为果蝇 Ets-like基因与D-Ets-2有大约60%的同源性，是 定位于染色体3R的97D。它制作了两份文字记录 胚胎期、蛹期和成虫期分别为2.3和2.0kb。果蝇 似乎很好地保存了ETS基因的3‘端区域 在至少两个不同的基因中，现在人们希望 果蝇将提供一种系统来确定这些基因的功能 基因。

Organisms from humans to Drosophila have been found to contain cellular sequences and transcripts that are homologous to viral- onc genes. The normal function of the genes encoded for by these cellular sequences is unknown. Only by understanding the normal function of these cellular genes (c-onc) will we have a possible means to understand how they can become transforming upon transduction by the virus. With this goal in mind, this study using Drosophila was begun. Cellular sequences homologous to the ets region of the chicken retrovirus, E-26, have been found in Drosophila in this laboratory. The characterized portion of this gene corresponds to the last two eons of the chicken c-ets-1 gene, and has over 90% homology at the predicted amino acid level. It is designated D-ets-2 and is localized on chromosome 3R at position 58 A/B, and it produces a single transcript of 4.7 Kb in all developmental stages. Low stringency hybridization of Drosophila genomic DNA shows several other bands that also hybridize with a viral ets probe, E1.28. Hybridization of a cDNA library under these conditions led to the isolation of a cDNA clone which shows considerable homology to v- ets but is not D-ets-2. This gene, called D-elg for Drosophila ets-like gene, has approximately 60% homology with D-ets-2 and is located on chromosome 3R at 97D. It produces two transcripts of 2.3 and 2.0 Kb in embryo, pupae, and adult stages. Drosophila appears to have conserved the 3' region of the ets gene very well in at least two different genes, and now it is hoped that Drosophila will provide a system to determine the function of these genes.