CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

果蝇 ETS 和 ETS 样基因的特征

• 批准号: 3916925
• 负责人: D K WATSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3916925
• 关键词: Drosophilidae; Retroviridae; complementary DNA; early embryonic stage; gene expression; genetic library; genetic transcription; genetic transduction; molecular cloning; nucleic acid hybridization; nucleic acid probes; oncogenes; protein sequence; viral carcinogenesis; virus genetics

Organisms from humans to Drosophila have been found to contain cellular sequences and transcripts that are homologous to viral- onc genes. The normal function of the genes encoded for by these cellular sequences is unknown. Only by understanding the normal function of these cellular genes (c-onc) will we have a possible means to understand how they can become transforming upon transduction by the virus. With this goal in mind, this study using Drosophila was begun. Cellular sequences homologous to the ets region of the chicken retrovirus, E-26, have been found in Drosophila in this laboratory. The characterized portion of this gene corresponds to the last two eons of the chicken c-ets-1 gene, and has over 90% homology at the predicted amino acid level. It is designated D-ets-2 and is localized on chromosome 3R at position 58 A/B, and it produces a single transcript of 4.7 Kb in all developmental stages. Low stringency hybridization of Drosophila genomic DNA shows several other bands that also hybridize with a viral ets probe, E1.28. Hybridization of a cDNA library under these conditions led to the isolation of a cDNA clone which shows considerable homology to v- ets but is not D-ets-2. This gene, called D-elg for Drosophila ets-like gene, has approximately 60% homology with D-ets-2 and is located on chromosome 3R at 97D. It produces two transcripts of 2.3 and 2.0 Kb in embryo, pupae, and adult stages. Drosophila appears to have conserved the 3' region of the ets gene very well in at least two different genes, and now it is hoped that Drosophila will provide a system to determine the function of these genes.

从人类到果蝇的生物体都含有 与病毒同源的细胞序列和转录本， onc基因。 这些基因编码的正常功能 细胞序列未知。 只有了解正常的 这些细胞基因（c-onc）的功能，我们将有可能 意味着了解它们如何在 通过病毒进行转导。 为了实现这一目标，这项研究 开始使用果蝇。 与鸡的ets区域同源的细胞序列 逆转录病毒，E-26，已在这个实验室的果蝇中发现。 该基因的特征部分对应于最后两个 与鸡c-ets-1基因的同源性在90%以上， 预测氨基酸水平。 它被命名为D-ets-2， 定位于染色体3R的58 A/B位，并产生一个 4.7Kb的单个转录本。 低 果蝇基因组DNA的严格杂交显示了几个 也与病毒ets探针E1.28杂交的其他条带。 在这些条件下的cDNA文库的杂交导致 分离显示与v-有相当同源性的cDNA克隆， 但不是D-ets-2。 这个基因，果蝇的D-elg ets样基因，与D-ets-2具有约60%的同源性， 位于染色体3R的97D处。 它产生两个转录本， 2.3胚胎期、蛹期和成虫期为2.0Kb。 果蝇 似乎很好地保守了ets基因的3 '区 至少有两种不同的基因，现在人们希望， 果蝇将提供一个系统来确定这些功能 基因.