CHARACTERIZATION OF DROSOPHILA MELANOGASTER--ETS AND ETS-LIKE GENES

果蝇 ETS 和 ETS 样基因的特征

• 批准号: 3838410
• 负责人: D K WATSON
• 金额: --
• 依托单位: DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3838410
• 关键词: Drosophilidae; carcinogenesis; developmental genetics; gene expression; genetic library; genetic mapping; molecular cloning; neoplasm /cancer genetics; nucleic acid sequence; oncogenes; protein structure function; protooncogene; transcription factor; transfection

D-elg (Drosophila ets-like gene) is a member of the Drosophila ets-related gene family that has extensive homology to the ets proto-oncogene. Drosophila represents an excellent system for the study of gene function because of the wealth of developmental, genetic, and molecular approaches available. D-elg serves as a promising gene to investigate ets-related gene function since it maps in a genetically well-characterized region of the Drosophila genome; this should facilitate the identification and analysis of D-elg mutants. Toward a goal of understanding the function of this ets-related gene in normal development, the molecular analysis of D- elg, a member of the Drosophila ets gene family, has been continued. Based on the characterization of cDNA and genomic sequences, the D-elg gene contains at least five exons and produces an mRNA with an open reading frame of 464 amino acids. Consistent with this analysis, in vitro translation of a near full-length D-elg cRNA yields a protein of about 56 kD. D-elg shows significant homology to other ets proteins in the amino- terminal A domain and strong homology in the carboxy-terminal ETS domain. The D-elg protein is most similar to the alpha subunit of the mouse GA binding protein, and thus may represent the Drosophila homolog of this mouse transcription factor. D-elg is expressed as a single transcript of 2.0 kb that is expressed throughout embryogenesis, as well as pupae and adult stages, but absent from larvae. D-elg is expressed in all embryonic cells prior to transcriptional activation and has no regional localization. The carboxy terminal end of the D-elg protein has been overexpressed and used to generate antibodies to D-elg.

D-elg（Drosophila ets-like gene）是果蝇ets相关基因家族的一员 与ets原癌基因具有广泛同源性的基因家族。 果蝇是研究基因功能的一个极好的系统 由于发育、遗传和分子方法的丰富， available. D-elg是研究ets相关性的一个有希望的基因 基因功能，因为它映射在一个遗传特征良好的区域， 果蝇基因组;这将有助于识别和 D-elg突变体的分析。 为了达到理解 这种与ets相关的基因在正常发育中，D- elg基因是果蝇ets基因家族的一员。 基于cDNA和基因组序列的表征， 基因包含至少五个外显子，并产生一个开放的mRNA。 464个氨基酸的阅读框架。 与该分析一致，在体外 近全长D-elg cRNA的翻译产生约56 kD。 D-elg与其它ets蛋白在氨基酸序列上有很大的同源性， 末端A结构域和羧基末端ETS结构域中的强同源性。 D-elg蛋白与小鼠GA的α亚基最相似 结合蛋白，因此可能代表果蝇同源物， 小鼠转录因子 D-elg表达为一个单一的转录本， 2.0 kb，其在整个胚胎发生中表达，以及puplatin和 成虫阶段，但没有幼虫。 D-elg在所有胚胎中表达， 在转录激活之前，细胞没有区域性的 本地化 D-elg蛋白的羧基末端已经被 过表达并用于产生针对D-elg的抗体。