通过蛋白质-蛋白质相互作用调节 ETS 功能-猫眼课题宝

通过蛋白质-蛋白质相互作用调节 ETS 功能

批准号：

3774880

负责人：

D K WATSON

金额：

$0.0万

依托单位：

DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS

依托单位国家：

美国

项目类别：

财政年份：

资助国家：

美国

项目状态：

未结题

起止时间：

至

来源：

https://reporter.nih.gov/project-details/3774880

关键词：

DNA binding protein chimeric proteins gene induction /repression genetic library genetic manipulation genetic regulation human genetic material tag human tissue intermolecular interaction molecular cloning nucleic acid probes nucleic acid sequence oncogenes oncoproteins protein engineering protein purification protein structure function transfection /expression vector

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中文摘要

翻译

在人类中发现了一个不断扩大的与et相关的基因家族

英文摘要

An ever-expanding family of ets-related genes has been found in the human genome, as well as in that of other species. Features of this ets family include a DNA-binding domain, as well as a helix-loop-helix motif, which has been postulated to be a site for protein-protein interaction. The goal of this project is to identify proteins which interact with individual ETS proteins, and thus give rise to functional differences among the members of the ets family. To this end, defined coding regions of the human ETS1 and ETS2 genes have been placed into prokaryotic pET vectors, which can be induced to overexpress the fusion protein, which then can be rapidly purified. These engineered proteins are radiolabelled and used to probe expression libraries to identify proteins with which ETS proteins interact. Preliminary results have revealed several clones which appear to interact with ETS1. This interaction will be further investigated to define the domains required for interaction. These methods will also be employed to identify proteins which interact with other members of the ets family.

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